云南芒康地区山脉阳坡面和阴坡面土壤微生物分布及药敏情况

Distribution and Drug Sensitivity of Mountain Soil Microorganisms at Markam District in Yunnan Province

目的对云南芒康地区山脉土壤中微生物数量、分布及药敏情况进行调查分析,为该地区感染性疾病的预防、诊断和治疗提供依据。方法选取云南芒康地区海拔相近的4个山脉的阳坡面和阴坡面作为采样点,按随机布点法采集土壤微生物,培养后应用全自动微生物鉴定仪进行细菌和真菌的分离鉴定,应用K-B法进行药敏试验,应用VITEK 2系统进行厌氧菌的鉴定和药敏试验。结果阴坡面微生物总数（7.34±0.59）×10^6cfu/g,明显高于阳坡面（5.71±0.55）×10^6cfu/g,差异有显著性（P〈0.05）;土壤中微生物以细菌为主（97.5%）,其中革兰氏阴性菌（56.5%）多于革兰氏阳性菌（36.7%）,厌氧菌占4.3%,而真菌数量较少（2.5%）。所分离出的绝大部分微生物对常用抗生素的敏感率均达100%。结论云南芒康地区山脉阳坡面和阴坡面土壤中微生物的数量与分布明显不同,但对常用抗生素均较敏感。

Objective To investigate the distribution and drug sensitivity rates of microorganisms in mountain soil, so as to provide a basis for the prevention, diagnosis and treatment of infectious disease at Markam district, Yunnan province. Methods We selected sunny slope and shady slope of 4 mountains as sampling points at Markam district. The soil sampling was collected as zigzag. After culture, bacteria and fungi were isolated and i- dentified by TREK DLAGNOSTIC SYSTEMS LTD automated microbial identification system. Susceptibility test was conducted by K-B method. Identification and susceptibility test of anaerobes was conducted by VITEK 2 System. Results The total amount of microorganism in shady slope [（7.34±0.59） x10^6 cfu/g] was significantly higher than that in sunny slope [ （5.71±0. 553 ） x 106 cfu/g] （P〈0.05）. Only 2.5% of soil microorganism was fungi, while 97.5% was mainly bacteria （ Gram-negative bacteria 56.5% , Gram-positive bacteria 36.7% , and anaerobes 4.3% ）. And most microorganisms were sensitive to commonly used antibiotics. Conclusion The amount of mountain soil mi- croorganisms in shady slope is higher than in sunny slope at Markam district. The most microorganisms seperated are sensitive to commonly used antibiotics.