摘要
TYRO3、AXL和MER受体是受体酪氨酸激酶亚家族之一,广泛地表达在哺乳动物神经、免疫、生殖、血液等系统多种细胞中,促进细胞存活、增殖和分化。本研究利用基因芯片技术筛选基因敲除的TYRO3^(^(-/-))AXL^(-/-)MER^(-/-)小鼠骨髓细胞中差异性表达的m RNA,并进行生物信息学分析。与正常小鼠骨髓细胞相比,TYRO3^(-/-)AXL^(-/-)MER^(-/-)小鼠骨髓细胞中差异表达基因探针共1 363条,其中表达上调的基因探针499条、下调的基因探针864条。GO功能富集分析发现上调的基因主要参与免疫反应,下调的基因主要参与造血。KEGG Pathway富集分析发现上调的基因主要参与细胞粘附分子和抗原呈递等信号通路。Real-time PCR验证5个差异基因,与芯片中表达变化趋势一致。因此TYRO3、AXL和MER受体共同参与调控机体的免疫反应和血细胞分化。
The receptors, TYRO3, AXL and MER, are one of tyrosine kinases receptor subfamilies, which are widely expressed in cells of nervous, immune, reproductive and hematopoietic systems of mammalian as well as promote cell survival, proliferation and differentiation. In this research, the differential expressed genes were screened from the bone marrow cells of TYRO3-/-AXL-/-MER-/-gene knockout mice by gene chip technique, and were analyzed by bioinformatics. Compared with the bone marrow cells of normal mice（wildtype）, total 1 363 differential expression gene probes of TYRO3-/-AXL-/-MER-/-in mice bone marrow cells were indentified which included 499 gene probes up-regulated and 864 gene probes down-regulated. Based on GO functional enrichment analyses, the up-regulated genes mainly involved in immune response and the down-regulated genes mainly involved in hematopoiesis.While KEGG Pathway enrichment analyses presented that the up-regulated genes involved in signaling pathways,such as cell adhesion molecules and antigen presentation. Five different expressing genes validated by Real-time PCR were in concordance with the changing trends detected by microarray chip. Therefore, TYRO3, AXL and MER receptors might participate in jointly regulating in immune response and hematopoiesis.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2016年第4期832-837,共6页
Genomics and Applied Biology
基金
河北省自然科学基金(H2013209194)
河北省高等学校科学技术研究项目(QN20131059)共同资助
