猪Cidec两种可变剪切体的发现及组织分布

Detection and Tissue Distribution of the Two Alternative Splicing of Cidec in the Pig

研究发现猪Cidec存在两种可变剪切体,分别为长亚型Cidce1和短亚型Cidec2。Cidec1翻译出的蛋白质有248个氨基酸,而Cidec2翻译出的蛋白质有238个氨基酸。通过定量PCR检测发现,在猪的肝脏和小肠里Cidec1为主要存在亚型,而在猪的肌肉和脂肪中Cidec2为主要亚型,且两亚型在三元杂猪(DLY)各组织中表达量也存在差异。根据不同亚型的组织分布差异推测Cidec1和Cidec2在脂肪代谢中发挥不同作用,后构建其表达载体和定位载体,为进一步研究蛋白功能和亚细胞定位提供后续实验材料。

This study showed that there were two alternatives splicing of Cidec in the pig, long subtypes Cidce1 and short subtypes Cidec2 respectively. Cidec1 translate 248 amino acids, and Cidec2 translate 238 amino acids.Through quantitative PCR detection, Cidec1 is the major subtype in the liver and small intestine, and the major subtype is Cidec2 in the muscle and fat, and there are differences between two isoforms in DLY. According to the distribution of different subtypes inference, Cidec1 and Cidec2 play different roles in the fat metabolism. We constructd the expression vector and fluorescence positioned vectors for further study on protein function and subcellular localization of subsequent experiment materials.