基于高通量测序的出芽短梗霉转录组学研究

Deep Sequencing-based Transcriptome Analysis of Aureobasidium pullulan

为获得出芽短梗霉合成普鲁兰多糖的转录组信息,揭示出芽短梗霉生长发育过程中转录组的整体表达特征,本研究采用Illumina Hi Seq 2500平台对出芽短梗霉swp 35进行高通量测序,结合生物信息学方法开展基因表达谱研究和功能基因预测。通过测序、组装共得到29 919条Transcript和20 312条UniGene。所有UniGene与NCBI的非冗余蛋白质数据库(Nr)进行相似搜索(E值<10^(-5)),结果一共有13 465个UniGene与数据库中的已知基因同源。此外,还对Uni Gene进行了GO、COG和KEGG的功能注释、分类及通路分析,获得一批与普鲁兰多糖合成相关的基因。通过高通量测序,获得了丰富的出芽短梗霉合成普鲁兰多糖的转录组数据,为深入研究出芽短梗霉合成普鲁兰多糖的代谢调控提供了有价值的数据。

To obtain t ranscriptome information of Aureobasidium pullulans, and reveal the overall expression characteristics of A. pullulans transcriptome during growth process. The transcriptome of A. pullulans was sequenced by Illumina Hi Seq 2500 platform, meanwhile, gene expression profiling and functional gene were predicted by using bioinformatics method. De novo assembly of ESTs data generated 29 919 Transcript and 20 312 Uni Gene. Similarity searches against the NCBI-Nr database revealed that 13 465 of these Uni Genes have significant matches（E-value〈10-5）. In addition, the Uni Genes were similarity searched against the GO, COG and KEGG databases, and annotated with gene functional descriptions, gene ontology terms or pathways. Then a number of genes associated with pullulan synthesis were obtained. By high-throughput deeply studying the theoretical basis of sequencing, we obtained abundant transcriptome information, which is very important databases for pullulan biosynthesis pathway and regulation.