长链非编码RNA CCAT1在肝细胞癌组织及细胞株中的表达及临床意义

Expression and clinical significance of long non-coding RNA CCAT1 in hepatocellular carcinoma tissues and cell lines

目的探讨长链非编RNA结肠癌相关转录子1(LncRNA-CCAT1)在肝细胞癌(HCC)组织及细胞株中的表达及临床意义。方法采用QPCR法检测CCAT1在4种肝癌细胞株(SMMC-7721、Hep3B、Hub7和HepG 2)、正常肝细胞株LO2和42例HCC组织及其癌旁组织中的差异性表达,分析CCAT1表达与HCC临床病理特征的关系(性别、年龄、甲胎蛋白、肿瘤大小、肿瘤数量、淋巴结转移、分化程度和TNM分期)。结果与正常肝细胞株LO2相比,CCAT1在SMMC-7721、Hep3B、Hub7和HepG 2细胞株中均呈高表达(P<0.05),其中在HepG 2细胞中表达水平最高。CCAT1在HCC组织中的表达水平高于其癌旁组织,差异有统计学意义(P=0.016);CCAT1在肝癌组织中的表达水平与肿瘤大小、甲胎蛋白、分化程度、TNM分期和淋巴结转移有关(P<0.05),而与性别、年龄、肿瘤数量无关(P>0.05)。结论 LncRNA CCAT1与HCC发生、发展及预后有关,其作用机制值得进一步研究。

Objective To explore the expression of long non-coding RNA of colon cancer associated transcript 1（ LncRNACCAT1） in hepatocellular carcinoma（ HCC） tissues and cell lines,as well as its clinical significance. Methods CCAT1 expression in 42 pairs of HCC tissues and paired adjacent normal tissues as well as 4 types of HCC cell lines（ SMMC-7721,Hep3 B,Hub7 and HepG 2） and normal hepatic cell line LO2 were tested by QPCR. The relationship between the expression of CCAT1 and common clinical pathological parameters（ gender,age,alpha-fetoprotein,tumor size,tumor number,lymph node metastasis,differentiation and TNM stage） were analyzed accordingly. Results Compared with normal hepatic cell line LO2,there was a high expression of CCAT1 in SMMC-7721,Hep3 B,Hub7 and HepG 2 cell lines with significant difference（ P〈 0. 05）,especially in HepG 2 cell line. The expression of CCAT1 in HCC tissue was higher than that in the adjacent tissues,and the difference was statistically significant（ P〈 0. 05）. The expression of CCAT1 in HCC was related with tumor size,alpha fetoprotein,differentiation,TNM stage and lymph node metastasis（ P〈 0. 05）,but not with age,gender and tumor number（ P〉 0. 05）. Conclusion LncRNA CCAT1 has a role in the occurence,development and prognosis of HCC,worthy of further research on its mechanism.