泰山磐石散对复发性流产小鼠母胎界面Th1/Th2细胞因子及妊娠预后的影响

Influence of Taishan Panshi San to Recurrent Spontaneous Abortion Mouse Maternal-fetal Interface Th1/Th2 Cytokines and Pregnancy Prognosis

目的:观察泰山磐石散对复发性流产小鼠母胎界面Th1/Th2细胞因子及妊娠预后的影响,为泰山磐石散临床应用提供新的实验依据。方法:采用经典造模方式DBA/2小鼠与CBA/J杂交,获得复发性流产小鼠模型,随机将与DBA/2小鼠合笼的60只CBA/J妊娠小鼠分为模型组、中药低剂量组、中药中剂量组、中药高剂量组和阳性对照组;与BALB/C合笼的10只CBA/J孕鼠作为正常妊娠模型。于妊娠14 d后处死孕鼠,观察小鼠胎盘丢失情况,并提取培养胎界母细胞,24 h后收集细胞上清液,酶联免疫吸附测定(ELISA)法检测上清液中肿瘤坏死因子α(TNF-α)、γ干扰素(IFN-γ)、白细胞介素4(IL-4)和IL-10含量。结果:经泰山磐石散治疗后,与模型组比较,复发性流产小鼠胎盘丢失率明显改善,母胎界面细胞上清液中Th1型细胞因子IFN-γ明显降低,Th2型细胞因子IL-4、IL-10明显升高,Th1/Th2免疫调节失衡明显改善,以中药高剂量组改善最为明显。结论:泰山磐石散能改善复发性流产小鼠胎盘丢失情况,其具体机制可能是通过调节Th1/Th2免疫调节平衡实现。

Objective：To investigate the effects of Taishan Panshi San on the maternal-fetal interface Th1/Th2 cytokines and pregnancy outcomes of recurrent abortion mouse, to provide a new experimental basis for clinical application of Taishan Panshi San. Methods：Classic modeling approach DBA/2 mice CBA/J hybridization, get recurrent abortion mouse models, 60CBA/J mice that randomly caged with DBA/2 mice were divided into model group pregnancy, low Chinese medicine dose group,middle Chinese medicine dose group, high Chinese medicine dose group and positive control group; 10 pregnant CBA/J mice mated with BALB/C as a normal pregnancy. 14 days after pregnancy pregnant rats were sacrificed to observe the mouse placenta ′s missing, and to extract oocytes cultured fetal community, 24 hours latter cell supernatants were collected, Elisa assay supernatant TNF-α, IFN-γ, IL-4, IL-10 content. Results：Compare with model group, the recurrent miscarriage mouse placenta loss ratio was significantly improved after treated with Taishan Panshi San, cytokine Th1 and IFN-γ were significantly decreased in cell supernatants of mother-fetal bound, Th2 cytokines IL-4 and IL-10 were significantly increased, Th1/Th2 immunoregulatory imbalance improved significantly, the high dose Chinese tradition medicine group was the most obvious. Conclusions：Taishan Panshi San can significantly improve the placenta lost situation of recurrent abortion mouse, the specific mechanism may be achieved by adjusting the balance of Th1/Th2 immune regulation.