生物信息学分析人C型凝集素16A的结构与功能

Bioinformatic Analysis of C- type Lectin Domain Family 16A

C型凝集素16 A基因(C-type lectin domain family 16A,Clec16a)是糖尿病、多发性硬化病等自身免疫性疾病的易感基因,但针对该基因功能的研究报道很少.通过生物信息学相关数据库和在线软件对CLEC16A蛋白分子的理化性质,亚细胞定位,跨膜区和信号肽,二级结构,结构域,磷酸化、泛素化、糖基化等蛋白翻译后修饰,以及蛋白相互作用网络等进行分析.结果表明,人Clec16a基因的共识编码序列为1053个氨基酸组成的多肽,是酸性不稳定的亲水蛋白,无跨膜区和信号肽,定位于多个亚细胞结构,其主要二级结构元件为α螺旋和随机卷曲,无已知的功能性结构域,存在磷酸化、泛素化、糖基化修饰位点.为深入研究CLEC16A功能及其参与的疾病分子机制提供一定的参考.

C- type lectin domain family 16 A is a susceptibility gene of autoimmune diseases such as diabetes and multiple sclerosis;however,its genetic function is rarely reported. In this article,we analyzed physical and chemical properties,the subcellular localization,transmembrane region and signal peptide,secondary structure,the conserved domains,protein modification after translation,and protein- protein interaction networks of CLEC16 A through bioinformatics. Results showed that the consensus coding sequence of clec16 a gene was a 1053 amino acids peptide. The hydrophilic protein was not stable,without transmembrane area and signal peptide,and located in multiple cellular structure. The main secondary structure elements were alpha helix and random curl,without known functional domains. Phosphorylation,ubiquitin,glycosylation modification sites existed. It provided certain reference for further research on molecular mechanism and functions of CLEC16 A participating in diseases.