延长制备时间对新鲜冰冻血浆和冷沉淀质量的影响

Effect of Prolonged Preparation time on the Quality of Fresh Frozen Plasma and cold Precipitation

目的研究和分析延长制备时间对新鲜冰冻血浆和冷沉淀质量的影响。方法收集新鲜冰冻血浆共40人份,制备的时间分别是6 h、8 h、10 h和12 h,将其平均分为对照组和观察组,对照组中的新鲜冰冻血浆的制备时间为10 h和12 h,观察组中的新鲜冰冻血浆的制备时间为6 h和8 h,两组均使用多美达速冻机对制备的新鲜冰冻血浆进行速冻,并用新鲜冰冻血浆进行冷沉淀的制备,检测两组新鲜冰冻血浆的凝血因子Ⅷ的含量,以及冷沉淀中纤维蛋白原和凝血因子Ⅷ的含量。结果观察组新鲜冰冻血浆的凝血因子Ⅷ含量,以及冷沉淀中的凝血因子Ⅷ的含量均高于对照组(P<0.05;而冷沉淀的纤维蛋白原含量的差异影响不大(P>0.05)。结论延长制备时间对新鲜冰冻血浆中凝血因子Ⅷ含量有着很大影响,而对纤维蛋白原的含量的影响则不大。

Objective To study and analysis to extend the time of preparation of impact on the quality of fresh frozen plasma and cryoprecipitate. Methods To collect fresh frozen plasma, a total of 40 doses, prep- aration time is 6 h,8 h,10 h and 12 h,its average can be divided into control group and observation group, control group of fresh frozen plasma preparation time for 10 h and 12 h ,the preparation of fresh frozen plasma in the observation group for 6 h and 8 h ,tome of quick-freezing machine should be used in two groups was car- i tied out on the preparation of fresh frozen plasma quick-frozen ,precipitation and the cold fresh frozen plasma preparation, detection of two groups of fresh frozen plasma clotting factor Ⅷ content, and fibrinogen in cryo Ⅷ content and blood coagulation factors. Results Observation group of fresh frozen plasma coagulation factor Ⅷ content, as well as cold precipitation of clotting factor Ⅷcontent were higher than control group （ P 〈 0. 05 ; and the difference of the fibrinogen content of eryo did not make any difference （P 〉 0. 05）. Conclusion To extend the time of preparation of fresh frozen plasma clotting factor Ⅷ content has great influence, while the impact on the content of fibrinogen.