红花黄色素对糖尿病肾病模型大鼠肾脏细胞血管紧张素Ⅱ1型受体的影响

Effects of safflower yellow on angiotensin receptor Ⅱ1 of kidney cells in diabetic nephropathy rats

背景:红花黄色素有治疗糖尿病肾病等作用,能够保护肾脏功能,减少损害,延缓或阻止糖尿病肾病的发展。目的:进一步验证红花黄色素对糖尿病肾病大鼠肾脏的作用以及其对肾脏细胞血管紧张素Ⅱ1型受体的表达的影响。方法:选择30只大鼠,随机分为3组,正常对照组、模型组和实验组,每组10只大鼠。实验组和模型组大鼠建立糖尿病肾病模型。建模成功后1周开始实验组大鼠每天给予红花黄色素注射液27.8 mg/kg腹腔注射,1次/d,模型组和对照组每天给予等量生理盐水腹腔注射,共给药23周。观察大鼠的各项肾脏相关生化指标、肾脏组织的病理学变化以及肾脏组织血管紧张素Ⅱ1型受体的表达情况。结果与结论:1实验组和模型组大鼠的肥大指数明显高于对照组(P<0.05)。2模型组的24 h蛋白尿、糖化血红蛋白、总胆固醇、三酰甘油、肌酐及尿素氮均高于对照组(P<0.05);实验组的24 h蛋白尿、糖化血红蛋白、总胆固醇及尿素氮均高于对照组(P<0.05);实验组的24 h蛋白尿、总胆固醇、三酰甘油、肌酐及尿素氮均低于模型组(P<0.05)。3模型组大鼠肾小球肥大,毛细血管的基底膜明显增厚,细胞增生,系膜增宽;对照组大鼠肾脏组织结构发现异常改变;实验组大鼠的肾脏组织病理学损害程度介于对照组和模型组之间。4实验组和模型组的肾脏组织血管紧张素Ⅱ1型受体表达水平高于对照组(P<0.05),实验组的肾脏组织血管紧张素Ⅱ1型受体表达水平低于模型组(P<0.05)。结果说明,红花黄色素对糖尿病肾病的肾脏具有保护作用,其作用机制可能与其阻断肾脏局部的肾素-血管紧张素系统有关。

BACKGROUND： Safflower yel ow is known to treat diabetic nephropathy, can protect kidney function, reduce lesions, delay or prevent the development of diabetic nephropathy. OBJECTIVE： To further verify the effects of safflower yel ow on the kidney in rats with diabetic nephropathy and the impact of safflower yel ow on the expression of renal cel receptor of angiotensin II1. METHODS： Thirty rats were equally and randomly divided into three groups： normal control group, model group and experimental group. In the experimental group and model group, rat models of diabetic nephropathy were established. At 1 week after model establishment, rats were daily intraperitoneally given safflower yellow injection 27.8 mg/kg, once a day. In the model and control groups, rats were daily intraperitoneally given an equal volume of saline. All rats were administered for 23 weeks. The kidney-related biochemical indicators and pathological changes in kidney tissue and the expression of angiotensin II1 type receptor on kidney tissue of rats were observed. RESULTS AND CONCLUSION： （1） Hypertrophy index of experimental group and model group was significantly higher than that of control group （P 〈 0.05）. （2） 24-hour proteinuria, glycosylated hemoglobin, total cholesterol, triglycerides, creatinine and urea nitrogen of model group were higher than that of control group （P 〈 0.05）. 24-hour proteinuria, glycated hemoglobin, total cholesterol and urea nitrogen in experimental group were higher than that of control group （P 〈 0.05）. 24-hour proteinuria, total cholesterol, triglycerides, creatinine and urea nitrogen in the experimental group were lower than that of model group （P 〈 0.05）. （3） Glomerular hypertrophy, thickening of capillary basement membrane, cell proliferation, and mesangial widening were detected in the model group. Abnormal changes in the kidney structure were found in the control group. The extent of damage of kidney of histopathology in the experimental group was between the control group and the model group. （4） The expression level of angiotensin receptor II1 of experimental group and model group was higher than that of control group （P 〈 0.05）. The expression level of angiotensin receptor II1 of experimental group was lower than that of model group （P 〈 0.05）. These results indicated that safflower yellow has a protective effect on diabetic nephropathy; the mechanism of action may be associated with blocking kidney partial renin-angiotensin system.