摘要
该文探讨了改良大鼠心肌梗死模型的制作方法。大鼠麻醉后,经口腔插管连接呼吸机,开胸结扎左冠状动脉前降支,28 d后颈动脉注入伊文思蓝,取出心脏,观察心脏外形和梗死区。结果表明,心肌梗死模型的成功率为87.5,存活率90;术后28 d,左心室明显扩大,梗死区心室壁变薄,颜色变白。该实验在于提供简化、易行、详细的心肌梗死模型的制作方法,供研究心肌梗死、心衰方向的初学者参考。
This paper discusses the preparation method of improving rat myocardial infarction model.Aftcr anaesthesia and oral trachea cannula,animal model was produced by ligation of the left anterior descending through left thoracotomy.Through carotid artery injecting Evans blue and taking out the heart,we can observe the heart share and infarction area after 28 days.The success rate of myocardial infarction model is 87.5,the survival rate is 90.28 days after surgey the left ventricle become large,the left ventricular wall of infarction area is thin,the color is white.This research provides a simple,easy,detailed method of making the myocardial infarction model by the experiment,for beginners reference.
出处
《实验科学与技术》
2016年第2期19-21,37,共4页
Experiment Science and Technology
基金
国家自然科学基金(81001497)
成都中医药大学实验技术项目(063040)
成都中医药大学校基金项目(030018060)
关键词
心肌梗死
模型
结扎
myocardial infarction
model
ligation
