影响铁皮石斛类原球茎诱导和增殖生长因素研究

Study on Influencing Factors for Induction and Proliferation of Protocorm-like Bodies of Dendrobium officinale

目的:提高铁皮石斛类原球茎诱导和增殖生长率。方法:以铁皮石斛幼芽为材料,采用1/2MS+2%蔗糖+10%香蕉泥+0.8%琼脂、p H值6.0为基本培养基。研究铁皮石斛类原球茎诱导的最佳激素浓度;类原球茎不同生理状态、接种外植体大小、培养天数、叠层培养方法对类原球茎增殖生长的影响。结果:类原球茎诱导添加6-BA 0.3 mg/L、NAA 0.2 mg/L为最佳浓度组合;颜色淡黄、疏松饱满的类原球茎增殖和增重迅速;最适的培养时间为45 d左右;相同生理状态的类原球茎以10个为一团接种,培养45 d增殖和增重最为显著,增殖率高达1008%,植株再生能力增强,平均每克类原球茎增殖后可再生植株1 132株;叠层培养上层类原球茎增殖和增重效果好,此法也可进行类原球茎复壮。结论:筛选出促进铁皮石斛类原球茎诱导最佳激素浓度组合和增殖生长技术参数,完善了铁皮石斛类原球茎诱导和增殖生长技术体系。

Objective： To improve the protocorms of Dendrobium officinale induction and proliferation growth rate. Methods： Dendrobium officinale plumule was selected as experimental material and 1 /2MS ＋ 2% sucrose ＋ 10% banana puree ＋ 0. 8% agar with p H value of 6. 0 was applied as minimal medium. The optimal hormone concentration for the induction of protocorm-like bodies of Dendrobium officinale was explored; effects of different physiological statuses of protocorm-like bodies,sizes of inoculated explants,days of cultured,and methods of laminated culture on the proliferation and growth of protocorm-like bodies were studied. Results： Addition of 6-BA0. 3 mg / L and NAA 0. 2 mg / L was the optimal concentration for the induction of protocorm-like bodies; protocorm-like bodies with light yellow color and loosened and plump form presented faster proliferation and weight gained; ten protocorm-like bodies with similar physiological statuses were inoculated as one group. The most significant proliferation and weight gained were observed after cultured for 45 d,the proliferation rate was up to 1008%. The plants presented strengthened regeneration capacity and the average amount of regenerated plants by each gram of protocorm-like bodies after proliferation was 1 132. In the process of laminated culture,protocorm-like bodies on the upper layer presented satisfactory condition of proliferation and weight gained,and this method was also applicable for rejuvenation of protocorm-like bodies. Conclusion： The optimal hormone concentration and proliferation growth technical parameters are screened out for promoting the Dendrobium officinale protocorms,which improved the Dendrobium officinale protocorms induction and technology system of proliferation growth.