槲皮素亚微乳在小鼠体内药代动力学及组织分布研究

Study on Pharmacokinetics and Tissue Distribution of Quercetin Submicroemulsion in Mice

目的:研究槲皮素注射液、槲皮素亚微乳注射剂静脉注射后在小鼠体内的药代动力学过程及组织分布。方法:采用反相高效液相色谱法测定小鼠血浆和组织中的槲皮素浓度。结果:槲皮素亚微乳剂在各组织中的分布顺序依次为肝>血>肾>脾>肺>心>脑,且其在血浆、肝、脾、肾、脑中的AUC0→t显著高于普通注射液(P<0.05),在肝、脑、脾、肾中的Rte、Re均大于1。结论:槲皮素亚微乳剂静脉注射液能明显改变槲皮素在小鼠血浆中的药代动力学特征,并具有明显的肝、脑、脾、肾组织中的靶向定位特征。

Objective： To study the pharmacokinetic and tissue distribution of quercetin injection and submicron emulsion after intravenous administration in mice. Methods： The concentration of quercetin in mice plasma and tissues were determined by RPHPLC. Results： Liver had the highest tissue concentration of quercetin submicron emulsion,followed by plasma,kidney,spleen,lung,heart and brain. And their had significantly increased compared with the AUC0→tof plasma,liver,spleen,kidney and brain after administration of quercetin injection（ P〈0. 05）. The Rte and Re of liver,brain and spleen were all larger than 1. Conclusion： Quercetin submicron emulsion significantly alters the plasma pharmacokinetic characteristics of quercetin after intravenous administration in mice. And it has good targeting location features in liver,brain,spleen and kidney.