血清TNF-α和sEC的表达与原发性肝癌侵袭和转移的关系

Study on the Relationship between the Expression of Serum TNF-α and s EC and Invasion and Metastasis of Primary Hepatic Carcinoma

目的探讨检测原发性肝癌患者手术治疗前后外周血肿瘤坏死因子-α(TNF-α)和血清可溶性E-钙粘蛋白(s EC)的表达及侵袭转移的关系。方法检测66例原发性肝癌患者手术前后及50例健康人TNF-α、s EC和AFP的水平,分析术前TNF-α、s EC和AFP水平与临床病理特征的关系。结果原发性肝癌患者术前TNF-α、s EC水平显著高于健康人,手术1周后TNF-α、s EC水平较术前显著下降。术前TNF-α水平与肿瘤分级、TNM分期密切相关(P<0.01),与肿瘤大小、有无门静脉癌栓、有无包膜相关(P<0.05),与年龄、性别、有无腹水无关(P>0.05)。术前s EC水平与肿瘤分级、TNM分期密切相关(P<0.01),与有无门静脉癌栓、有无包膜、有无腹水相关(P<0.05),与年龄、性别、肿瘤大小无关(P>0.05)。结论 TNF-α和s EC参与原发性肝癌的侵袭和转移,在肝癌的恶性进展中起着重要作用,可作为一种肝细胞癌预后和复发预测因子。

Objective To investigate the relationship between the expression of serum tumor necrosis factor-alpha（ TNF-α） and soluble E-cadherin（ s EC） and invasion and metastasis of primary hepatic carcinoma（ PHC）. Methods The levels of TNF-α,s EC and AFP in serum of 66 PHC patients and 50 healthy controls were tested ELISA,and its relationship with clinical and pathological characteristics were analyzed. Results The levels of TNF-α and s EC in PHC patients before operation were significantly higher than those in controls,which declined significantly one week after operation. The of TNF-α level before operation showed significant correlation with Edmondson grade,TNM staging,tumor size,the carcerous thrombi within the portal vein and the capsule of carcinoma（ P〈0. 05 or P〈0. 01）,but had no correlation with age and sex of patients or ascites（ P〈0. 05）. The of s EC level before operation showed significant correlation with Edmondson grade,TNM staging,ascites,the carcerous thrombi within the portal vein and the capsule of carcinoma（ P〈0. 05 or P〈0. 01）,but had no correlation with age and sex of patients or the tumor size（ P〉0. 05）. Conclusion The expression of TNF-α and s EC involves in the process of invasion and metastasis of PHC. It plays important roles in malignancy of PHC,which may be a predictive factor for prognosis and recurrence of PHC.