氧化应激与高同型半胱氨酸血症的关系探讨

Study of relationship between oxidative stress and hyperhomocysteinemia

目的:探讨氧化应激与高同型半胱氨酸血症(HHcy)的关联性,浅析HHcy可能致病机制。方法:于2014-10-2015-05连续收集67例HHcy患者,另筛选34例健康体检者(对照组)。分别进行血清超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)、丙二醛(MDA)水平检测,并进一步分析SOD、T-AOC、MDA与Hcy之间的相关性。结果:HHcy患者血清MDA、T-AOC水平均高于正常对照组(U=471.5,P<0.01;U=186,P<0.01),而SOD含量低于正常对照组(U=194,P<0.01)。HHcy病情中度组相比轻度组间,仅MDA水平升高(U=245,P=0.006),其他指标在2组间差异无统计学意义(P>0.05)。此外,研究结果表明Hcy水平与MDA、TAOC呈正相关性,与SOD呈负相关性。结论:HHcy患者体内氧化作用与抗氧化作用失衡,导致清除自由基能力明显降低,致使自由基在体内大量蓄积,进一步促进细胞或组织的损伤,通过对SOD、T-AOC、MDA氧化应激指标的检测,可为HHcy患者的致病机制、预防及抗氧化治疗奠定基础。

Objective：To explore the relationship between oxidative stress and hyperhomocysteinemia（HHcy）,and analyze the possible pathogenic mechanisms of HHcy.Method：Sixty-seven HHcy patients and 34 healthy subjects（healthy control group）were collected from October of 2014 to May of 2015.The level of serum superoxide dismutase（SOD）,total antioxidant capacity（T-AOC）and malondialdehyde（MDA）were detected,and the correlation between SOD,T-AOC,MDA and Hcy were further analyzed.Result：The levels of serum MDA and T-AOC were both higher than those of healthy control group（U =471.5,P〈0.01;U =186,P〈0.01）.The level of serum SOD was lower than that of healthy control group（U=194,P〈0.01）.Compared severe group with mild group in HHcy patients,the level of serum MDA was only increased（U=245,P=0.006）.The other indexes were not significantly different between the two group（P〈0.05）.In addition,we found that the correlation of Hcy with MDA,T-AOC was positive（P〈0.05）and the correlation of Hcy with SOD was negative（P〈0.01）.Conclusion：In HHcy patients,the oxidation and antioxidant imbalance leads to that the ability to scavenge the free radicals is evidently reduced,leading to the free radical accumulation,which destroys the cells and tissue.By detecting of the oxidative stress indicators of SOD,T-AOC and MDA,we can provide the foundation for the pathogenic mechanism,prevention and antioxidant therapy in HHcy patients.