摘要
目的:明确云南种植的粗茎秦艽斑枯病的发生规律及病原菌的分类地位,以期为该病的防治提供依据。方法:通过田间调查掌握该病发生的基本规律,经组织分离、柯赫氏法则验证获得病原菌,依据真菌的形态特征和ITS序列特征确定其分类地位。结果:基于真菌形态和ITS序列特征,粗茎秦艽斑枯病病原被鉴定为小孢壳针孢Septoria microspora,该病在云南粗茎秦艽主要种植区普遍发生。结论:小孢壳针孢Septoria microspora是粗茎秦艽斑枯病病原菌。
Objective： To identify the pathogen of Gentiana crassicaulis leaf spot in Yunnan province. Methods： The pathogen was isolated from leaf spot of G. crasicaulis with Potato Dextrose Agar medium,tested and verified with Koch ＇s postulates,and identified Based on morphological and ITS DNA sequence. Results： The pathogen was identified as S. microspora. Conclusion： S. microspora is the pathogen of G. crasicaulis leaf spot.
出处
《中国现代中药》
CAS
2016年第3期292-295,共4页
Modern Chinese Medicine
基金
云南省科技创新强省计划项目(2014AE015)
云南省科技创新人才计划(2015HB102)
关键词
粗茎秦艽
斑枯病
病原鉴定
小孢壳针孢
Gentiana crasicaulis
leaf spot
pathogen identification
Septoria microspora
