菊花破壁饮片的HPLC指纹图谱研究

Study on HPLC Fingerprint of Cell Wall-Broken Decoction Pieces of Chrysanthemi Flos

目的:建立菊花破壁饮片HPLC指纹图谱,并分析破壁饮片成品与其中间品、原料的化学成分相关性,为菊花破壁饮片整体质量评价提供依据。方法:采用高效液相色谱法,十八烷基硅烷键合硅胶为填充剂的色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.5%磷酸溶液为流动相进行梯度洗脱,检测波长:348 nm,柱温:35℃。结果:建立了菊花破壁饮片的HPLC指纹图谱,得到了15个共有特征峰,11批样品的相似度达0.98以上,方法学考察结果符合指纹图谱技术要求。结论:所建立的方法稳定、可靠、重复性好,可用于菊花破壁饮片质量控制和综合评价。

Objective： To establish the HPLC fingerprint of Cell Wall-Broken Decoction Pieces of Chrysanthemi Flos,and analyze the relativity between its decoction pieces,broken powder and broken particle,helping to evaluate its quality comprehensively. Methods： RP-HPLC method was performed on an Agilent C18（ 250 mm × 4. 6 mm,5 μm） column with a gradient elution composed of acetonitrile-aqueous solution containing 0. 5% phosphoric acid. The column temperature was set at35 ℃,while the detective wavelength was set at 348 nm. Results： The chromatographic fingerprint common pattern was established. Fifteen mutual peaks were obtained from the chromatograms of eleven batches of samples. Conclusion： The method with good reproducibility is reliable and stable,which is feasible for quality control of cell wall-broken decoction pieces of Chrysanthemi Flos.