坎地沙坦对慢性束缚应激诱导的大鼠抑郁样行为的作用

Effect of candesartan on chronic restraint stress-induced depression-like behaviors in rats

目的观察坎地沙坦对慢性束缚应激(CRS)诱导的大鼠抑郁样行为的改善作用,探索抗抑郁治疗的新靶点。方法雄性Wistar大鼠24只,随机分为3组:空白对照组(n=8)、束缚应激组(n=8)和坎地沙坦组(n=8)。通过旷场实验(OFT)、糖水偏爱实验(SPT)及强迫游泳实验(FST)评估大鼠抑郁样行为。用ELISA法检测血浆促肾上腺皮质激素(ACTH)及血管紧张素Ⅱ(AngⅡ)水平。结果坎地沙坦组与应激组比较,OFT中直立次数及水平位移显著升高(P=0.000),SPT中对糖水偏好程度增加(P=0.027),FST中不动时间缩短(P=0.000),血浆ACTH明显下降(P=0.000)。结论坎地沙坦可改善CRS诱导的大鼠抑郁样行为。

Objective To observe the effect of candesartan on improving chronic restraint stress （CRS） -induced depression-like behaviors in rats and explore novel targets for the treatment of depression. Methods Twenty- four male Wistar rats were randomly assigned to control group （ n =8）, stressed group （ n =8）, and candesartan group （n=8）, respectively. Open field test （OFT）, sucrose preference test （SPT）, and forced swimming test （FST） were performed to evaluate depression-like behaviors in rats. ELISA was used to detect adrenocorticotropin hormone （ACTH） and aniotensin Ⅱ （Ang Ⅱ） levels. Results Compared with the stressed group, rearing frequency and horizontal moving distance in OFT in the candesartan group significantly increased （P=0. 000）, sucrose preference percentage in SPT elevated （P=0. 027）, immobility time in FST reduced （P=0. 000）, and plasma ACTH level significantly decreased （P=0. 000）. Conclusion Candesartan can improve CRS-induced depression-like behaviors in rats.