临床细菌检验中PCR检验法的准确性分析

Analysis of the Accuracy of PCR Test in Clinical Bacteria Test

目的综合分析PCR检验法在临床细菌检验中的准确性,为疾病的临床诊断和治疗提供科学的参考依据。方法我院2014年1月~2015年6月收治的阴道炎患者中随机选取106例进行研究,所有患者均分别采用细菌培养法（A组）和PCR检验法（B组）进行细菌检验,对比两种方法的检验结果。结果经比较,B组的检出率为84.91%,高于A组的59.43%,组间差异具有统计学意义（P〈0.05）。B组的肠球菌、戛纳杆菌和棒状杆菌检出率分别为15.09%、34.91%和34.91%,均高于A组的6.60%、26.42%和26.42%,组间各指标差异均具有统计学意义（P〈0.05）。结论与细菌培养法比较,PCR在临床细菌检验中具有更高的检出率和准确率,可以为阴道炎的临床诊疗提供可靠依据。

Objective Analysis of the accuracy of PCR test method in clinical bacteria inspection and provide a scientific reference for clinical diagnosis and treatment of diseases. Methods From January 2014 and June 2015,106 cases of vaginitis patients treated in our hospital were randomly selected for study,all patients respectively by bacterial culture method（group A）and PCR test method（group B） and comparison of the two methods of test results. Results After comparison,the detection rate of B group was 84.91%,significantly higher than that of group A of 59.43%,there was significant difference between the groups（P〈0.05）. Group B of enterococci,Cannes coli and Corynebacterium were 15.09%,34.91% and 34.91%,were significantly higher than those in a group A of 6.60%,26.42% and 26.42%,the differences had statistical significance（P〈0.05）. Conclusion Compared with the bacterial culture method,PCR has a higher detection rate and accuracy in clinical bacterial examination. It can provide reliable evidence for clinical diagnosis and treatment of vaginitis,and has obvious advantages and high clinical application value.