WCG无血培养基在幽门螺旋菌分离培养中的初步应用

Preliminary Application of Non-blood WCG Culture Medium in Helicobacteria of pylorus Isolating Culture

用ＷＣＧ无血培养基与ｓｋｉｒｒｏｗ（以下简称ＳＫ）培养基，同时进行已知幽门螺旋菌（Ｈｐ）的培养及胃活检标本中Ｈｐ的分离试验。结果表明：３２株已知菌在ＷＣＧ培养基上生长的菌落比ＳＫ上生长的丰茂，特点明显，菌体形态及生化反应和耐受试验一致。６５份胃活检标本用ＷＣＧ分离阳性者４１例，阳性率为６３．１％，用ＳＫ分离阳性者３９例，阳性率６０．０％，分离阳性率ＷＣＧ与ＳＫ无明显差异（Ｘ２＝０．１３，Ｐ＞０．０５）。实验证明ＷＣＧ无血培养基可代替含血培养基用于Ｈｐ分离培养。

Culture of given helicobacteria of pylorus(HP)and isolation test of HP samples of biopsy were conducted both in culture medium of non-blood WCG and blood skirrow(SK). The results show that 32 strain of given HP colony produced in WCG were abundant and more distinguished than those in SK,and the somatic morphosis,biochemieal reaction and tolerant test were consistent,Of the 65 samples of gastric biopsy, 41 were isolated in WCG (63.1%),and 39 cases isolated in SK(60.0%),There was no significant difference (X2=0.13 ,P>0.05) between the two groups on positive rate. This study demonstrates that blood culture may be replaced by non-bloodculture.