云南产螺旋藻多糖的分离纯化与分析

Isolation, Purification and Analysis of the Polysaccharide from Spirulina Platensis

云南程海湖工业化养殖的螺旋藻（干品）经热水提醇沉，ｓｅｖａｇ法脱蛋白，透析，再经ＤＥＡＥ－ｓｅｐｈａｄｅｘ－Ａ２５与ｓｅｐｈａｄｅｘＧ－２００柱层析纯化，得螺旋藻多糖（ＰＳＰ）。用纸层析，柱层析及电泳检查纯度，表明ＰＳＰ为单一组分。ＰＳＰ经ＵＶ－３００扫描，无核酸（２６０ｎｍ）及蛋白质（２８０ｎｍ）特征吸收峰。ＩＲ－４００扫描，出现典型多糖吸收峰。经纸层析及气相色谱分析表明ＰＳＰ组成单糖为Ｌ－岩藻糖、Ｄ－甘露糖、Ｄ－半乳糖、Ｄ－葡萄糖和葡萄糖醛酸。ＰＳＰ平均分子量约为１６６００。

The Polysaccharide(PSP)was isolated form Spirulina platensis raised in Yunnan. PSP was further purified by DEAE-sephadex-A 25 and sephadex G-200 Its homogeneity was examined by paper chromatography, sephadex G-200 chromatography and PAGE respectively. The monosaccharide composition of PSP was identified by paper and gas chromatography. The results show that PSP is composed of L-fucose, D-mannose,D-galactose,D-glucose and glucuronic acid, its molecular weight estimated to be 16600.