摘要
采用酶法和碘淀粉法同时对84例健康成人的血、尿样品进行淀粉酶含量检测,并对两种方法作了比较。结果表明:两种方法所得参考值差异显著(P<0.01),无论操作上还是结果的判断上酶法都较碘法优越,且操作简便,快速易行,化学计量关系明确、可大,是目前比较理想、值得临床推广的检测方法。
In this paper, PNPG7 blocked substrate and iodine-amylase colorimetry were simultaneously used to measure the amylase content in the blood and urine samples of 84 healthy adults, and a contrast between the two methods was also made. The results revealed that the difference between two methods was significant and PNPG7 blocked substrate was much better than iodine-amylase colorim etry(P< 0.01).The operation of PNPG7 blocked substrate is simpler and easier to be made and the chemical measurement with this method is definitive and reliable as well. Therefore PNPG 7 blocked substrate is considered an ideal test at present,and valuable to be popularized in clinical practice.
关键词
淀粉酶
酶偶联法
碘淀粉法
Amylase
PNPG7 blocked substrate
Iodine-amylase colorimetry