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酶偶联测定法和碘-淀粉比色法在淀粉酶测定中的应用比较

A Contrast between PNPG7 Blocked Substrate and Iodine-Amylase Colorimetry in the Measurement of Amylase
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摘要 采用酶法和碘淀粉法同时对84例健康成人的血、尿样品进行淀粉酶含量检测,并对两种方法作了比较。结果表明:两种方法所得参考值差异显著(P<0.01),无论操作上还是结果的判断上酶法都较碘法优越,且操作简便,快速易行,化学计量关系明确、可大,是目前比较理想、值得临床推广的检测方法。 In this paper, PNPG7 blocked substrate and iodine-amylase colorimetry were simultaneously used to measure the amylase content in the blood and urine samples of 84 healthy adults, and a contrast between the two methods was also made. The results revealed that the difference between two methods was significant and PNPG7 blocked substrate was much better than iodine-amylase colorim etry(P< 0.01).The operation of PNPG7 blocked substrate is simpler and easier to be made and the chemical measurement with this method is definitive and reliable as well. Therefore PNPG 7 blocked substrate is considered an ideal test at present,and valuable to be popularized in clinical practice.
出处 《大理学院学报(医学版)》 1996年第2期9-10,共2页
关键词 淀粉酶 酶偶联法 碘淀粉法 Amylase PNPG7 blocked substrate Iodine-amylase colorimetry
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