摘要
目的：探讨ＲＴ－ＰＣＲ在病毒性心肌炎中的诊断价值。方法：选用柯萨奇Ｂ组病毒（ＣＶＢ）保守区５′－非编码区的组特异性引物，对４２例临床拟诊为病毒性心肌炎的患者血清行ＲＴ－ＰＣＲ，以检测ＣＶＢ－ＲＮＡ，同时采用间接ＥＬＩＳＡ检测血清中ＣＶＢ－ＩｇＭ。结果：心肌炎患者血清中ＣＶＢ－ＲＮＡ的检出率为６６７％（２８／４２），而ＩｇＭ的检出率为５２４％（２２／４２）。结论：ＲＴ－ＰＣＲ和ＥＬＩＳＡ都是特异、敏感的诊断方法，并且ＲＴ－ＰＣＲ比ＥＬＩＳＡ敏感（Ｐ＜００５）
Objective:In order to test the applicability of reserve transcription polymerase chain reaction (RT-PCR) in diagnosis of viral myocarditis in patients. Methods: To select a pair of primers in conserved 5’-noncoding region (5’-NCR) of Coxsachievirus B (CVB) amplified CVB-RNA In the meantime, CVB-IgM was detected by indirect enzyme-linked-immunoabsorbent assay(ELISA).Results:28 of 42 CVB-RNA were positive,while 22 of 47 CVB-IgM were positive. Conclusion: RT-PCR is a kind of specific, sensitive method in diagnosis of viral myocarditis. It is more sensitive than ELISA is.
