健脾祛痰化瘀中药对高脂雄性巴马小型猪血清TC、TG、HDL、LDL、脑组织ABCA1、ApoE及Aβ影响随机平行对照研究

Jianpi Qutan Huayu Chinese medicine on high fat male Obama miniature pig serum TC, TG, HDL, LDL, brain tissue of ABCA1, Apo E and the influence of A beta random parallel control study

[目的]观测健脾祛痰化瘀中药对高脂雄性巴马小型猪血清TC、TG、HDL、LDL、脑组织ABCA1、Apo E及Aβ影响。[方法]使用随机平行对照方法,55只雄性巴马小型猪适应性饲养1周,随机数字表法分为正常组5只,手术组50只(球囊挤压过程死亡8只,存活42只),存活42只小型猪重新随机数字表法分6组,7只/组:模型组(n=7)、健脾祛痰化瘀组(n=7)、健脾祛痰组(n=7)、健脾组(n=7)、祛痰化瘀组(n=7)、西药组(n=7)。正常组基础饮食,手术组球囊挤压术后继续给予高热量-高脂-适量胆盐喂饲,日进食量为上周所称体重的3%,自由饮水。并于24周末进行取材,观察血清TC、TG、HDL、LDL的变化和脑组织中ABCA1、Apo E及Aβ的含量变化。[结果]造模后检测各组血脂均明显高于正常组(P<0.01)。与正常组相比,模型组TC,TG,HDL,LDL的含量均明显升高(P<0.01)。与模型组相比,健脾祛痰组、祛痰化瘀组、健脾祛痰化瘀组TC,TG,LDL的含量均明显降低(P<0.01),HDL含量均明显升高(P<0.01)。健脾祛痰组调节TC、HDL、LDL的能力强于祛痰化瘀组和健脾祛痰化瘀组,而健脾祛痰化瘀组调节TG的能力强于健脾祛痰组和祛痰化瘀组。与正常组相比,模型组脑组织ABCA1、Apo E及Aβ的含量均明显升高(P<0.01)。与模型组相比,健脾祛痰组、祛痰化瘀组、健脾祛痰化瘀组脑组织ABCA1、Apo E的含量均明显升高(P<0.01),Aβ的含量明显降低(P<0.01)。与健脾祛痰化瘀组相比,健脾祛痰组、祛痰化瘀组脑组织ABCA1、Apo E的含量降低(P<0.01),Aβ的含量升高(P<0.01)。疗效由强及弱为健脾祛痰化瘀组>健脾祛痰组、祛痰化瘀组。[结论]健脾祛痰化瘀中药通过调整高脂雄性巴马小型猪血清TC、TG、HDL、LDL、脑组织ABCA1、Apo E及Aβ含量,以减少脑内Aβ的沉积,从而降低阿尔茨海默病的发生与发展。

[Objective] Observation of Jianpi Qutan Huayu Chinese medicine on high fat male Obama miniature pig serum TC, TG, HDL, LDL, brain tissue of ABCA1, Apo E and a beta influence. [Method] Using random parallel control method, 55 male Bama miniature pig 1 week adaptive feeding, the random number table method were divided into three groups： normal control group（n=5）, operation group 50（balloon extrusion process and 8 rats died, only 42 survived）, survival 42 pigs to the random number table method was divided into six groups, 7 rats / group： model group（n=7）, invigorating the spleen and removing phlegm and blood stasis group（n=7）, Jianpi Qutan group（n=7）, invigorating the spleen group（n=7）, eliminating phlegm and removing blood stasis group（n=7）, western medicine group（n=7）. Normal group based diet, surgery group after balloon extrusion to continue to give high calorie- high fat- the amount of bile salt feeding, daily intake of 3% of the weight of the last week, free drinking water. At the weekend of 24, the changes of serum TC, TG, HDL, LDL and the content of ABCA1, Apo E and A in the brain tissues were observed. [Results] After making model, the blood lipids were significantly higher than the normal group（P〈0.01）. Compared with the normal group, the contents of TC, TG, HDL and LDL in model group were significantly increased（P〈0.01）. Compared with the model group, Jianpi Qutan group, eliminating phlegm and removing blood stasis group, spleen and removing phlegm and blood stasis group TC, TG, LDL levels were significantly lower（P〈0.01）, the content of HDL were significantly increased（P〈0.01）. Jianpi Qutan group to adjust the TC, HDL, LDL and strong in eliminating phlegm and removing blood stasis group, invigorating the spleen and removing phlegm and blood stasis group, and invigorating the spleen and removing phlegm and blood stasis group regulation ability of TG strong in Jianpi Qutan group and phlegm stasis group. Compared with the normal group, the contents of ABCA1, Apo E and A in model group were significantly increased（P〈0.01）. Compared with the model group, the content of Jianpi Qutan group, eliminating phlegm and removing blood stasis group, spleen and removing phlegm and blood stasis group, the brain ABCA1 and apo E were significantly increased（P〈0.01）, the content of a beta decreased significantly（P〈0.01）. Compared with the spleen and removing phlegm and blood stasis group, reduce the content of Jianpi Qutan group, eliminating phlegm and removing blood stasis group, brain ABCA1 and apo E（P〈0.01）, the content of a beta increased（P〈0.01）. The effect of strong and weak spleen and removing phlegm and blood stasis group, dispelling phlegm and removing phlegm and blood stasis group. [Conclusion] Jianpi Qutan Huayu Chinese medicine on high fat male Obama miniature pig serum TC, TG, HDL, LDL, brain tissue of ABCA1, Apo E and a beta content adjustment, to reduce brain Abeta deposition, thereby reducing the occurrence and development of Alzheimer＇s disease.