鼎药方枳白黄的GC-MS测定和交互移动窗口因子法分析

Analysis of triherbal recipe fructus aurantii-atractylodes macrocephala-radix scutellariae by gaschromatography-mass spectrometry and alternative moving window factor

鼎药方是仅含3个单味药的方剂,是药对方基础上的又一种复方基本形式,比药对能更好地揭示复方的配伍机制,采用GC-MS法分离测定鼎药方枳白黄(FA-AM-RS)、单味药枳壳(FA)、白术(AM)和黄芩(RS)的挥发油成分,结合交互移动窗口因子法(AMWFA)对其共有组分进行比较分析,得到鼎药方及其3个单味药的纯色谱曲线和质谱图;用质谱库结合保留指数进行定性,采用总体积积分法进行定量。研究结果表明:鼎药方枳白黄、单味药枳壳、白术和黄芩分别定性出89,47,46和17种化学成分,分别占挥发油总体积的84.18%,92.64%,92.29%和80.99%;鼎药方与枳壳共有组分39个,与白术共有组分37个,与黄芩共有组分8个,其挥发油组分主要来自于枳壳和白术。

Considering that triherbal recipe is the prescription of three herbs and fundamental form of compound prescription that is used to study herbal-pair prescriptions, and it can more effectively reveal compatibility mechanism of compound prescription than herbal-pair prescriptions, the volatile components in triherbal recipe Fructus AurantiiAtractylodes Macrocephala-Radix Scutellariae（FA-AM-RS） were analyzed, single herb FA and AM and RS were performed with two-dimensional gas chromatography-mass spectrometry（GC-MS） data coupled with alternative moving window factor analysis（AMWFA）. The results show that, by means of these methods two-dimensional data, 89, 47, 46 and 13 volatile chemical components in essential oil of triherbal recipe FA-AM-RS, FA, AM and RS are determined qualitatively and quantitatively, accounting for 84.18%, 92.64%, 92.29% and 80.99% total contents of essential oil of triherbal recipe FA-AM-RS, FA, AM and RS, respectively. There are 39 common active constituents between triherbal recipe FA-AM-RS and single herb FA, and 37 common active constituents between FA-AM-RS and single herb AM, 8 common active constituents between FA-AM-RS and single herb RS. The major volatile chemical components in FA-AM-RS are mainly from that of single herbs FA and AM.