培养基调节对黑木相思增殖的影响

Effect of different medium on multiplication culture of Acacia melanoxylon

以增殖率较低的21号黑木相思无性系继代苗为材料,希望通过培养基调节,提高生产效率,为推动规模化发展打下基础。通过单因子试验,以苗高、新芽数、愈伤质量和苗木生物量为衡量指标,筛选出最佳的大量元素、6-BA、IBA及糖用量。结果表明：培养基中大量元素含量对愈伤质量和生物量影响显著,以MS大量最好;6-BA含量对苗高、新芽数、愈伤质量都产生显著影响,以不加6-BA苗高最高,6-BA 0.5 mg/L获得最多5.4个新芽/苗,6-BA 1.0 mg/L愈伤质量最大,IBA浓度只对新芽数产生显著影响,以IBA 0.5 mg/L新芽数最多,糖对所有指标产生显著影响,当糖20 g/L时,苗木生长最高,糖20~30 mg/L时,新芽数、愈伤质量和生物量最大。

Through improving the basic medium to increase the productivity of No. 21 clone of Acacia melanoxylon which has relatively low proliferation rate, to suit to the large scale production. The optimum concentration of macro elements, 6-BA, IBA and sugar in medium were selected by single factor experiment based on the data of growth and proliferation of shoots, callus quality and biomass of seedlings. The results showed that： In medium, the MS macro elements had significant effect on the callus quality and biomass. The favorable concentrations for growth was without 6-BA, for the multiplication was0.5 mg/L, in which had 5.4 shoots per plantlet, for the callus yield was 1.0 mg/L. The medium supplemented with 0.5 mg/L IBA was suitable for the growth of shoots. Sugar ranged from 20 to 30 g/L was the best for the growth and proliferation of shoot, callus quality and biomass of the plantlets.