支原体肺炎患儿体内氧化应激变化及盐酸氨溴索对其影响的研究

Research of changes and influence by ambroxol hydrochloride on oxidative stress in children with mycoplasma pneumoniae pneumonia

目的研究肺炎支原体肺炎(以下简称支原体肺炎)患儿血浆及诱导痰氧化应激水平的变化,探讨盐酸氨溴索治疗支原体肺炎患儿对氧化应激的影响。方法 80例支原体肺炎患儿(支原体肺炎病例组),随机分为常规治疗组及干预组,各40例。常规治疗组予常规治疗,干预组予常规+盐酸氨溴索治疗。选取同期本院20例健康体检儿童血样标本作为对照组。以健康体检儿童为空白对照,检测常规治疗组和干预组患儿治疗1 d及治疗5、10 d的静脉血及诱导痰检测氧化产物丙二醛(MDA)、酶性抗氧化剂超氧化物歧化酶(SOD)水平。结果 1与对照组儿童相比,支原体肺炎病例组患儿治疗1 d血浆SOD水平下降、血清MDA水平升高(P<0.01)。2治疗5 d后常规治疗组与干预组患儿血浆、诱导痰SOD水平均无明显回升,两组差异无统计学意义(P>0.05);治疗10 d后,两组患儿血浆、诱导痰SOD水平均回升,干预组更明显(P<0.05或P<0.01)。3治疗5 d后两组患儿诱导痰MDA水平比较差异无统计学意义(P>0.05);治疗10 d后两组诱导痰MDA水平均明显下降,且干预组更显著(P<0.01)。结论支原体肺炎患儿体内存在明显氧化应激反应,盐酸氨溴索可以有效降低全身及肺脏局部氧化应激反应。

Objective To resesarch changes of oxidative stress lvels in plasma and induced sputum of children with mycoplasma pneumoniae pneumonia （mycoplasma pneumonia）, and to investigate influence by ambroxol hydrochloride on oxidative stress in children with mycoplasma pneumonia. Methods A total of 80 children with mycoplasma pneumonia （mycoplasma pneumonia group） were randomly divided into conventional treamtent group and intervention group, with 40 cases in each gorup. The conventional treatment group received conventional treatment, and the intervention group received conventional treatment ＋ ambroxol hydrochloride. Another 20 healthy children at the same period were taken as control group, and they were taken as blank control to detect oxidation products methane dicarhoxylic aldehyde （MDA） and enzymatic antioxidant superoxide dismutase （SOD） levels in venous blood and induced sputum of the conventional treatment group and the intervention group in 1, 5 and 10 d of treatment. Results （~） Compared with the control group, the mycoplasma pneumonia group had decreased plasma SOD level and increased serum MDA level in 1 d of treatment （P〈0.01）. （~） Both conventional treatment group and intervention group had no obvious improvements of SOD levels in plasma and induced sputum in 5 d of treatment. Their difference had statistical signficance （P〉0.05）. After 10 d of treatment, both groups had increased SOD in plasma and induced sputum, while the intervention group had more remarahle increase. （P〈0.05 or P〈0.01）. （~ There was no statistically significant difference of MDA level in induced sputum after 5 d of treatment between the two groups （P〉0.05）. After 10 d of treatment, both groups had obviously decreased MDA level in induced sputum, and the intervention group had more remarkable decrease （P〈0.01）. Conclusion Oxidative stress is obvious in children with myeoplasma pneumonia, and ambroxol hydrochloride can effectively decrease systematic and lung oxidative stress. This method is worth clinical application.