囊虫病快速免疫色谱测试法的建立和初步应用

STUDIES ON THE ESTABLISHMENT AND PRELIMINARY APPLICATION OF IMMUNOCHROM ATOGRAPHIC TEST FOR DIAGNOSIS OF CYSTICERCOSIS

目的 建立一种敏感、特异、简便快速的囊虫病免疫诊断方法。方法 以胶体金标记抗猪囊尾蚴抗原单克隆抗体 ,将另一抗猪囊尾蚴抗原单克隆抗体在硝酸醋酸混合纤维素膜上固定成一条线 ,制成免疫测试条。待测抗原与金标单克隆抗体结合后沿着硝酸醋酸混合纤维素膜移动 ,与膜上的特异性单克隆抗体结合形成肉眼可见的褐红色线。结果 当猪囊尾蚴囊液抗原量为 5 ng时 ,测试条仍呈阳性反应。检测脑囊虫病患者脑脊液 (CSF)的阳性率为 82 .6 0 % ,检测健康人 CSF的阴性符合率为 10 0 %。将本法检测 CSF结果与 EL ISA比较 ,两方法的敏感性和特异性相似。结论 本方法敏感、特异 ,操作简便快速 ,试剂稳定 ,重复性好 。

Objective To establish a simple,sensitive and reliable diagnostic method for cysticercosis.Methods One colloidal gold labled McAb against cyst fluid antigen of cysticercus was used as probe,another McAb and cyst fluid antigen of cysticercus were absorbed on cellulose membrane to serve as detecting and control line respectively to be made the immunochromatographic trips for the detection of cysticercus antigen. Antigen-antibody reaction was completed on the membrane during the solution absorbing process. The result could be read with the naked eyes. Results The minimal antigen of the test detection was 5 ng. The sensitivity and specificity of the test to detecting circulating antigen(CA) in cerebrospinal fluid(CSF) of patients with neurocysticercosis was 82.60% and 100% respectively. The test was easy to perform and the results can be obtained within 5-12 mimutes, immunochromatographic trips can store at 4℃ more than 5 months ,3 neurocysticercosis CSF samples were detected for 3 times repeatedly and the results were same. This test had a similar sensitivity and specificity with ELISA. Conclusion This assay was sensitive,specific and simple to perform, it is suitable for detecting cysticercus antien.