混凝土修复功能菌Bacillus cohnii DSM6307芽孢萌发条件探究

Study on the Condition of Spore Germination of Concrete Self-healing Bacterium Bacillus Cohnii DSM6307

目的:研究具有矿化能力的科氏芽孢杆菌(Bacillus cohnii,DSM6307)芽孢的萌发条件。方法:运用光吸收法对芽孢的萌发结果进行检测。结果:最适萌发缓冲液为Na2CO3/Na HCO3,芽孢萌发率为66.16%;萌发剂为肌苷,芽孢萌发率为83.89%,肌苷最适浓度为15 mmol/L;热激温度60℃,热激时间5 min,芽孢萌发率达到90.85%;而营养底物的加入,也促使芽孢迅速萌发,变成营养体。结论:萌发缓冲液、萌发剂的种类及浓度、热激温度及时间、营养底物均能影响芽孢的萌发率。

Objective： The condition of spore germination of Bacillus cohnii DSM6307 with mineralizationability was studied. Method：The method of light absorption was used to evaluate the spore germinationcondition. Result： The spore germination ratio was 66.16% with Na2CO3/NaHCO3 as the optimumgermination buffer; inosine was the optimum germinant, and spore germination ratio was 83.89% at theoptimal concentration of 15mmol/L; with 60℃ and heat shock 5min, spore germination ratio reached90.85%; the addition of nutritional substrates also prompted spore germination rapidly. Conclusion： Thespore germination ratio was affected by the germination buffer, the kind of the germinant, the time andtemperature of heat shock and the nutritional substrates.