干旱胁迫对3种木本植物酶活性的影响

Effects of Drought Stress on Activity of Enzyme for Three Kinds of Woody Plant

为研究植物对干旱胁迫的适应能力,采用人工模拟干旱胁迫的方式,以金叶莸、圆柏和忍冬为材料,分析其过氧化氢酶、过氧化物酶和多酚氧化酶在干旱处理下活性的变化。结果表明：3种绿化植物过氧化氢酶活性的变化随着干旱胁迫的加剧总体趋势大致相同,在处理的第1~10天酶活性缓慢下降,处理的第10~20天酶活性变化剧烈,第10~14天,3种绿化植物酶活性急速上升,并在14d时达到最大值,14~20d酶活性又大幅度下降。过氧化物酶活性的变化趋势也大致相同,从第1~14天都呈现缓慢波动上升的趋势,14~20天,3种植物的酶活性都急速上升,圆柏的酶活性最高。3种植物在活性氧防御系统中,3种酶协同作用,但前期起主要作用的是过氧化物酶和多酚氧化酶,过氧化物酶起互补作用,在重度胁迫下发挥主要作用。

In order to study adaptation of plant on drought stress,adopting manual simulation drought stress,Caryopteris×dandonensis‘worcester Gold＇,Sabina chinensis and Lonicera japonica as materials,changes of activities for catalase,peroxidase polyphenol oxidase under drought treatment were analyzed.Result shows that the general trend of changes of activities for catalase of three kinds of landscaping plant is approximately the same with the exacerbate of drought stress;enzyme activity declines slowly from the first to the tenth days.Enzyme activity changes severely from the 10 th to the 20 th day;enzyme activity of three kinds of landscaping plant rises rapidly during 10-14 d,being the maximum value at the 14 th day;enzyme activity dropped significantly during 14-20d;changing trend of activities for catalase are approximately the same,appeared upward trend of slow fluctuations during 1-14d;enzyme activity of three kinds of plant rises rapidly during 14-20 dand the activity of Sabina chinensis is the highest.In defense systems of reactive oxygen,three kinds of enzyme synergy,peroxidase and polyphenol play a major role in prophase;the oxidase,which plays a complementary role and,play a major role under severe stress.