血管紧张素(1-7)对氧化低密度脂蛋白诱导大鼠主动脉内皮细胞损伤的作用机制

Mechanisms of Angiotensin( 1-7) on Aortic Endothelial Cell Injury Induced by Oxidized Low Density Lipoprotein in Rats

目的观察血管紧张素(1-7)[Ang(1-7)]对氧化低密度脂蛋白(ox-LDL)诱导的大鼠主动脉内皮细胞(SVAREC)血凝素样氧化型低密度脂蛋白受体1(LOX-1)、细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)表达水平的影响,探讨其可能作用机制。方法将大鼠随机分为ox-LDL组和ox-LDL+Ang(1-7)组。实验通过Cell Counting Kit-8(简称CCK-8)检测细胞增殖情况。通过荧光实时定量PCR法检测LOX-1、VCAM-1、ICAM-1mRNA的表达水平。结果 ox-LDL可以抑制SVAREC细胞的增殖,并呈剂量依赖性,与对照组比较有统计学意义(P<0.05)。ox-LDL+Ang(1-7)组可以使SVAREC细胞生长抑制率降低,并呈剂量依赖性,与对照组比较有统计学意义(P<0.05)。随着ox-LDL浓度的升高,LOX-1、ICAM-1及VCAM-1 mRNA表达量上升,并呈剂量依赖性,与对照组比较有统计学意义(P<0.05),加入Ang(1-7),随着Ang(1-7)的浓度升高,LOX-1、ICAM-1及VCAM-1mRNA表达量下降。结论 ox-LDL可以损伤SVAREC细胞,并使LOX-1、ICAM-1及VCAM-1mRNA表达量上升,Ang(1-7)抑制ox-LDL的上述反应,并且可能通过LOX-1、ICAM-1及VCAM-1mRNA发挥作用。

Objective To investigate the effects of angiotensin (1 7)[Ang (1 7)]on expression levels of lectin like oxidized low density lipoprotein receptor 1 (LOX 1),intercel ular adhesion molecule 1(ICAM 1)and vascular cel adhesion molecule 1 (VCAM 1)in SV40 transformed aortic rat endothelial cel s (SVAREC)induced by oxidized low density lipoprotein(ox LDL),and to explore its possible mechanism.Methods Cultured endothelial cel s of rats were randomly divided into ox LDL group and the ox LDL+ Ang (1 7)group.The cel proliferation was detected by experimental Cel Counting Kit (CCK 8).The expression levels of LOX 1,ICAM 1,VCAM 1 mRNA were detected by fluorescent quantitative reverse transcription polymerase chain reaction (RT PCR)method.Results Ox LDL could inhibit SVAREC cel proliferation in a dose dependent manner,and there was statistical y significant compared with the control group (P<0.05).Compared with control group,SVAREC cel growth inhibition rate was reduced in Ox LDL+ Ang(1 7)group.With the increase of ox LDL concentration,LOX 1,ICAM 1 and VCAM 1 mRNA expression in-creased,and in a dose dependent manner,and there was statistical y significant compared with the control group(P<0.05).With the increase of Ang(1 7),LOX 1,ICAM 1 and VCAM 1 mRNA expression decreased in Ox LDL+ Ang(1 7)group.Conclu-sion SVAREC ox LDL can damage cel s and LOX 1,ICAM 1 and increased VCAM 1mRNA expression and Ang(1 7)inhibi-tion of ox LDL of the reaction,and through the LOX 1,ICAM 1 and VCAM 1mRNA play a role.