葡萄籽原花青素对糖尿病大鼠肾脏GLUT2及肾功能的影响

Efficacy of grape seed procyanidins on renal tissue GLUT2 and renal function in diabetic rats

目的:观察葡萄籽原花青素对糖尿病大鼠肾组织GLUT2及肾功能的影响。方法:将SD雄性大鼠随机分为对照组、糖尿病模型组、两个葡萄籽原花青素组。两个葡萄糖花青素组分别给予200mg·kg^(-1)·d^(-1)、400mg·kg^(-1)·d^(-1)灌胃,对照组和模型组灌等体积的生理盐水,8周后测定各组大鼠血NO、Ccr、尿蛋白定量,并处死大鼠取肾检测肾脏肥大指数,以免疫组化和免疫荧光技术检测肾组织GLUT2的表达。采用酶联免疫吸附法检测血清白细胞介素8、转化生长因子。结果:与对照组比较,糖尿病模型组大鼠肾脏GLUT2表达明显增加,NO水平、Ccr、尿蛋白定量、IL-8、TGF-β、肾脏肥大指数显著升高(P<0.05),NO/ET显著降低(P<0.05);与模型组比较,葡萄籽原花青素组大鼠肾脏GLUT2表达明显减少,NO水平、Ccr、尿蛋白定量、IL-8、TGF-β、肾脏肥大指数活性显著降低(P<0.05),NO/ET显著增高(P<0.05)。结论:葡萄籽原花青素能够降低肾脏GLUT2表达,在一定程度上改善DN模型大鼠肾脏损伤。

Objective To observe the effect of grape seed procyanidins on renal tissue GLUT2 and renal function in diabetic rats.Methods： SD male rats were randomly divided into the control group, the diabetes model group, and two grape seed procyanidins groups（the low dose group received 200mg·kg-1·d-1, the high dose group received 400mg·kg-1·d-1）. Two grape seed procyanidins groupswere respectively treated with corresponding dosage. The control group and the diabetes model group were given equal volume of saline.In 8 weeks, the blood NO, Ccr and urine protein quantitative were measured in each group. After rats were killed, kidney hypertrophyindex was detected; and renal tissue GLUT2 was detected by immunofluorescence technique. IL-8, TGF were detected by enzyme linkedimmunosorbent assay. Results： Compared with the control group, renal tissue GLUT2 expression was significantly increased in two grapeseed procyanidins groups, levels of NO, Ccr, urine protein quantitative, IL-8, TGF-β and kidney hypertrophy index were significantlyincreased （P〈0.05）. NO/ET was significantly lower （P〈0.05）. Conclusion： Grape seed procyanidins can reduce the expression of renaltissue GLUT2 and improve the renal damage of DN model partly.