大鼠急性脑缺血及再灌注时白细胞介素(IL-6、IL-8)的变化及意义

Change and Meaning of Interleuking(IL-6、IL-8) During the Acute Cerebral Ischemin and reperfusion of Rats

目的 :探讨大鼠急性脑缺血及再灌注后白细胞介素 (IL - 6、IL - 8)含量的变化。方法 :制备大鼠急性脑缺血及再灌注模型 ,采用酶联免疫吸附 (ELISA)法检测血清IL - 6、IL - 8含量。结果 :在脑缺血及再灌注过程中 ,IL - 6、IL - 8水平显著高于对照组 (P <0 .0 1) ,并且IL - 6水平有着逐步升高的现象 ,而IL -8在脑缺血后 2 0min及再灌注 1h后其含量呈持续性显著增高 (P <0 .0 1) ;再灌注 1～ 48h内虽有所降低 ,但仍维持在一个相当高的水平。结论 :IL - 6、IL -

Objective: To study the change of interleuking(IL-6?IL-8)during the acute cerebral ischemin and reperfusion of rats. Methods: Acute cerebral ischemin and reperfusion rat model was established. By using ELISA,the levels of IL-6?IL-8 in serum were measured. Result:The serum levels of IL-6?IL-8 were significantly higher than those in normal controls(P< 0.01). Levels of IL-6 increased gradaully,levels of IL-8 decreased during 1～48 hours,but still maintained a very higher level. Conclousion:IL-6 and IL-8 is involved in the pathogenesis of cerebral ischemia and reperfusion.