摘要
目的研究普伐他汀对新生大鼠成骨细胞的作用。方法用3-（4,5-二甲基噻唑-2）-2,5-二苯基四氮唑溴盐法（MTT）、4-硝基苯磷酸二钠法（PNPP）、茜素红染色法分别检测普伐他汀对成骨细胞的活力,碱性磷酸酶（ALP）活力及骨矿化结节面积的作用。结果普伐他汀在10-8~10-4 g/ml能促进成骨细胞增殖、提高成骨细胞碱性磷酸酶活性和促进新生大鼠成骨细胞骨结节形成。结论普伐他汀能促进成骨细胞增殖、分化及矿化。
Objective To research effect by pravastatin for osteoblast in infant rats. Methods 3-（4, 5-dimethylthiahiazo-2）-2, 5-phenytetrazoliumromide（MTT）, 4-nitrophenyl phosphate（PNPP） and alizarin red staining were applied respectively to detect activity of pravastatin for osteoblast, activity of alkaline phosphatase（ALP）, and effect of bone mineralization nodule area. Results Pravastatin in concentration of 10-8~10-4 g/ml accelerated osteoblast multiplication, and improve activity of osteoblast ALP, and promote osteoblast bone nodule formation in infant rats. Conclusion Pravastatin can accelerate multiplication, differentiation and mineralization in osteoblast.
出处
《中国现代药物应用》
2016年第10期278-281,共4页
Chinese Journal of Modern Drug Application
