小檗碱吴茱萸碱配伍对高脂血症模型大鼠肝脏HMG-CoA还原酶和L-FABP mRNA表达的影响

Effects of berberine combined with evodiamine on liver HMG- CoA reductase and L- FABP mRNA expression levels in a rat model of hyperlipidemia

目的:观察小檗碱和吴茱萸碱配伍对高脂血症模型大鼠肝脏HMG-CoA还原酶和L-FABP基因表达的影响,探讨黄连吴茱萸配伍发挥降脂作用的机制。方法:将40只健康雄性SD大鼠随机分为正常对照组,模型对照组,辛伐他汀组,小檗碱与吴茱萸碱配伍高、低剂量组,每组8只;使用高脂乳剂建立大鼠高脂血症模型,以18sRNA作为内参基因,RT-PCR技术检测各组大鼠肝组织中HMG-CoA还原酶和L-FABP mRNA相对表达水平。结果:小檗碱和吴茱萸碱配伍能显著降低高脂血症模型大鼠肝脏HMG-CoA还原酶和L-FABP mRNA表达,且高剂量组效果更优。结论:黄连吴茱萸降血脂有效成分小檗碱、吴茱萸碱配伍可降低高脂血症模型大鼠的血脂水平,其作用机制可能与抑制肝脏HMG-CoA还原酶和L-FABP基因表达有关。

Objective： To observe the effects of berberine combined with evodiamine on liver HMG-CoA reductase and L- FABP mRNA expression levels in a rat model of hyperlipidemia and to investigate the lipid- lowering mechanism. Methods： A total of 40 healthy male Sprague- Dawley rats were equally and randomly divided into normal control group,model control group,simvastatin group,and low- and high- dose berberine combined with evodiamine groups. The rat model of hyperlipidemia was established using high- fat emulsion. The relative expression levels of liver HMG- CoA reductase and L- FABP mRNA were measured using RT- PCR with 18 sRNA as a reference gene in these different groups. Results： Berberine combined with evodiamine significantly reduced the HMG- CoA reductase and L- FABP mRNA levels,and the high- dose group had greater reductions in the two indices. Conclusion：Berberine combined with evodiamine can reduce blood lipid level in the rat model of hyperlipidemia and its mechanism may be related to the inhibition of liver HMG- CoA reductase and L- FABP mRNA expression levels.