痛泻要方对溃疡性结肠炎模型大鼠血清IL-12含量及NPY水平影响的研究

Effects of Tongxieyaofang on serum levels of interleukin- 12 and neuropeptide Y in rats with ulcerative colitis

目的:探讨痛泻要方对溃疡性结肠炎(UC)模型大鼠IL-12及神经肽(NPY)的影响。方法:将SD大鼠随机分为空白组、模型组、柳氮磺嘧啶(SASP)组及痛泻要方高、中、低剂量组,每组各10只;采用TNBS/乙醇溶液灌肠法制备溃疡性结肠炎大鼠模型,运用ELISA法测定各组血清中细胞因子IL-12、神经肽Y(NPY)的含量。结果:与空白组比较,模型组血清IL-12、NPY水平均显著上升(P<0.01),各治疗组较模型组血清IL-12、NPY水平均明显下降(P<0.01)。结论:痛泻要方能通过下调IL-12、NPY的表达,调节肠道异常免疫反应,维持Th1、Th2的平衡,抑制炎症反应,修复溃疡,进而防治UC。

Objective： To investigate the effects of Tongxieyaofang on the serum levels of interleukin- 12（ IL-12） and neuropeptide Y（ NPY） in rats with ulcerative colitis（ UC）. Methods： Sprague- Dawley rats were randomly divided into blank group,model group,salicylazosulfapyridine（ SASP） group,and high-,medium-,and low- dose Xongxieyaofang groups,with 10 rats in each group. TNBS / alcohol solution was given to establish the rat model of UC,and ELISA was used to measure the serum levels of IL- 12 and NPY in each group. Results： Compared with the blank group,the model group had significant increases in the serum levels of IL- 12 and NPY（ P〈0. 01）. After treatment,all treated groups had significant reductions in the serum levels of IL- 12 and NPY compared with the model group（ P〈0. 01）. Conclusion： Tongxieyaofang can reduce the serum levels of IL- 12 and NPY,regulate abnormal intestinal immune response,maintain the balance between Th1 and Th2 cells,inhibit inflammatory response,repair ulcers,and thus prevent and treatment UC.