摘要
A crude protease produced from Planomicrobium sp. L-2 is described, and its effectiveness as an additive in liquid detergent evaluated. We isolate the protease-producing Planomicrobium sp. L-2 from the gastrointestinal tract of Octopus variabilis. At least three caseinolytic protease clear bands were observed in zymogram analysis. The crude alkaline protease was highly tolerant of a pH range from 7.0 to 9.0, and temperatures to 50℃ after incubation for 1 h. Proteolytic enzymes were stable towards three surfactants （5% Tween 80, 1% Triton X-100 and 0.05% SDS） and an oxidizing agent （1% hydrogen peroxide）, in addition to being highly stable and compatible with popular commercial laundry powered detergent brands available in China. Our study demonstrates the potential these proteases have for development into novel classes of detergent additive. This study also suggests that the gastrointestinal tract of Octopus variabilis may be a rich source of commercially valuable strains of enzvme.
A crude protease produced from Planomicrobium sp.L-2 is described,and its effectiveness as an additive in liquid detergent evaluated.We isolate the protease-producing Planomicrobium sp.L-2 from the gastrointestinal tract of Octopus variabilis.At least three caseinolytic protease clear bands were observed in zymogram analysis.The crude alkaline protease was highly tolerant of a p H range from 7.0 to 9.0,and temperatures to 50°C after incubation for 1 h.Proteolytic enzymes were stable towards three surfactants(5% Tween 80,1% Triton X-100 and 0.05% SDS) and an oxidizing agent(1% hydrogen peroxide),in addition to being highly stable and compatible with popular commercial laundry powered detergent brands available in China.Our study demonstrates the potential these proteases have for development into novel classes of detergent additive.This study also suggests that the gastrointestinal tract of Octopus variabilis may be a rich source of commercially valuable strains of enzyme.
基金
Supported by the Natural Science Foundation of Shandong Province(No.ZR2011CM023)
the High-Level Personnel Research Foundation of Qingdao Agricultural University(No.631431)
