蟾毒灵对人脑胶质瘤U251细胞生长增殖的影响

Influence of Bufalin on the Growth and Proliferation of Human Glioma Cell Line U251

目的:探讨蟾毒灵(Bufalin)对人脑胶质瘤U251细胞生长增殖的影响。方法:采用四甲基偶氮蓝(MTT)比色法检测Bufalin对人脑胶质瘤U251细胞增殖的影响;倒置显微镜观察各组细胞数目、形态及活性的变化;膜联蛋白(Annexin V)碘化丙啶(PI)标记法检测其对细胞凋亡的影响。结果:不同浓度Bufalin对U251细胞增殖活性具有不同程度的抑制作用,在0.001~10μmol·L^(-1)浓度范围内呈剂量和时间依赖性;与对照组相比,Bufalin给药组对U251细胞的凋亡率均显著增高(P<0.01)。结论:Bufalin可显著抑制U251细胞的生长增殖,并在一定范围内呈浓度和时间依赖性,诱导其凋亡。

Objective：To explore the effects of Bufalin on the growth and proliferation of human glioma cells U251.Methods：Methyl thiazolyl tetrazolium（MTT） assay was used to detect the effect of Bufalin on the proliferation of human glioma cells U251.An inverted microscope was used to observe the changes of cell number,morphology and activity.Annexin V/PI was used to measure the induction of cell apoptosis caused by Bufalin.Results：Bufalin at different concentrations（0.001-10.0μmol·L^-1） inhibited the proliferation of U251 cells in a dose and time-dependent manner.Compared with that of the control group,the apoptosis rate of Bufalin group was increased significantly（P〈0.01）.Conclusion：Bufalin can inhibit the growth and proliferation of U251 cells in a dose and time-dependent manner,and induce the apoptosis of U251 cells.