HIV基因芯片的初步研究

Preliminary study of development of gene chips for HIV diagnosis

目的建立HIV基因检测芯片的分析技术。方法分离HIV1U26942基因限制性显示片段作探针,应用PixSys 5500点样仪将探针打印在氨基包被的玻片上制作基因芯片。然后与随机引物荧光标记的HIV样品进行杂交,以分析限制性显示基因片段的杂交动力学。经杂交后清洗和干燥,扫描芯片进行检测。结果对基因检测芯片的制作与检测的实验条件进行了初步研究并筛选了12个限制性显示基因探针。结论建立的检测芯片的实验方法可行且较特异。

Objective To study the technology for establishing DNA chips for the diagnosis of HIV. Methods HIV 1U26942 DNA fragments were isolated by restriction display-PCR (RD-PCR) and printed onto aminosilane-coated glass slides by Pixsys 5500 arrayer as probes to prepare the gene chips. HIV samples, after labeled with Cy3, were hybridized with the microarray followed by scanning for analysis of hybridization kinetics of the RD fragments. Results The experimental condition for preparing the gene chips was investigated and 12 RD fragments were screened as probes for further study. Conclusion The technique established in this study for preparing DNA chips is specific and applicable.