人脐带间充质干细胞对耐亚胺培南铜绿假单胞菌耐药性形成的影响

Effect of human umbilical cord mesenchymal stem cells on the development of the drug resistance of imipenem-resistant Pseudomonas aeruginosa

目的探讨人脐带间充质干细胞(h UCMSCs)体外对耐亚胺培南铜绿假单胞菌(IRPA)耐药性形成及对Opr D2基因的影响。方法本实验设立3个组,实验组为h UCMSCs组,对照组为细胞对照组(即人肺成纤维细胞组,NHLF组)和空白对照组。次抑菌浓度肉汤诱导PA耐药传导过程中,h UCMSCs组和NHLF组分别加入其与PA共培育所得的上清液,空白对照组加入细胞培养液,观察3组诱导耐药所需代数以及抑菌圈的大小。诱导耐药前后分别采用K-B法及real-time PCR法测定PA对常见抗菌药物的敏感性及Opr D2基因的表达量。结果经次抑菌浓度的亚胺培南诱导后,h UCMSCs组PA耐药性的出现较NHLF组及空白对照组延迟。NHLF组和空白对照组PA于诱导的第17代出现亚胺培南耐药,而h UCMSCs组PA于第19代出现耐药性。real-time PCR结果显示,诱导耐药后PA中Opr D2表达量较诱导前出现减少或消失。其中h UCMSCs组PA Opr D2的表达量减少至诱导耐药前的10.96%,而NHLF及空白对照组Opr D2无表达,即诱导后出现Opr D2基因缺失。结论人脐带间充质干细胞具有延迟PA耐药性形成的作用,其机制可能是通过分泌抗菌肽LL-37和人β防御素-2从而抑制Opr D2表达的减少,而外膜蛋白Opr D2表达量减少或缺失是引起PA对亚胺培南耐药的原因。

Objective To investigate the effect of human umbilical cord mesenchymal stem cells（h UCMSCs） in vitro on the development of the drug resistance of imipenem-resistant Pseudomonas aeruginosa and on Opr D2 gene. Methods This experiment included three groups： experiment group（h UCMSCs group）, control group（cells control group, namely normal human lung fibroblast, NHLF group） and blank control group. During the conductive process of Pseudomonas aeruginosa（PA） drug resistance induced by sub-inhibitory concentrations of broth, the h UCMSCs group and the NHLF group were added with the supernatants obtained from their joint cultivation with PA, respectively; while the blank control group was administrated with cell culture medium. The numbers of generations required by induced drug resistance in the three groups and the sizes of inhibition zones were observed. The sensitivity of PA to common antibacterial agents and the expression quantity of Opr D2 gene were determined by K-B method and real-time PCR method. Results Through the induction of sub-inhibitory concentration of imipenem, the occurrence of PA drug resistance in the h UCMSCs group was relatively later than that of the blank control group. Imipenem drug resistance appeared in passage 17 of PA induced in the NHLF group and the blank control group, while it occurred in passage 19 in the h UCMSCs group. The results of real-time PCR showed that after the induction of drug resistance, the expression quantity of Opr D2 in PA decreased or disappeared compared with that before the induction. The expression quantity of Opr D2 in PA in the h UCMSCs group was reduced to 10.96% of the induction, while there were no Opr D2 expression in the NHLF and the blank control group, Opr D2 gene deletion occurred after the induction. Conclusions h UCMSCs could delay the formation of PA＇s drug resistance. This mechanism is probably through reducing the inhibition of the expression of Opr D2 by secreting antimicrobial peptide LL-37 and human β-defensin 2（HBD-2）, and the decrease or deletion of the expression of outer membrane protein Opr D2 is the cause for PA＇s drug resistance to imipenem.