摘要
目的探讨纤溶酶原激活物抑制剂1(PAI-1)基因启动子区4G/5G多态性与浙江地区汉族多囊卵巢综合征(PCOS)发病、胰岛素抵抗及肥胖的关系。方法选择浙江地区汉族PCOS患者120例和健康对照者102例,分别测量两组身高、体重、腰围,并计算BMI和腰臀比;采用PCR技术检测两组PAI-1基因启动子区4G/5G多态性分布;采用ELISA法检测PCOS患者空腹胰岛素,采用葡萄糖氧化酶法检测PCOS患者空腹血糖,并计算胰岛紊抵抗指数(Homa-IR)。再根据BMI、Homa-IR将PCOS患者分为肥胖组(54例)与非肥胖组(66例),以及胰岛紊抵抗组(49例)与非胰岛素抵抗组(71例),分别比较两组基因型频率。结果 PCOS患者组PAI-1基因启动子区4G/4G基因型频率明显高于健康对照组(P<0.05)。PCOS患者PAI-1基因启动子区4G/4G基因型频率为胰岛素抵抗者、非肥胖者分别高于非胰岛素抵抗者、肥胖者(P<0.05)。结论 PAI-1基因启动子区4G/5G多态性与浙江地区汉族PCOS发病、胰岛寨抵抗及肥胖有关;其中4G/4G基因型可能是PCOS发病、PCOS患者胰岛素抵抗的危险因素,且可能影响非肥胖型PCOS患者病情发展。
Objective To investigate the correlation of 4G/5G polymorphism of plasminogen activator-1 （PAI-1） gene promoter region with polycystic ovary syndrome （PCOS） in Zhejiang Han population. Methods One hundred and twenty patients with PCOS and 102 healthy women （control group） were enrolled from Zhejiang Han population. Body mass index （BMI） and waist-to-hip ratio （WHIR） were determined. The 4G/5G polymorphism of the PAI-1 gene promoter region was amplified by the polymerase chain reaction （PCR）. Blood samples of PCOS patients were obtained, fasting insulin was detected by ELISA and fasting glucose was measured by glucose oxidase method, and insulin resistance index （Homa-IR） was calculated. Based on the BMI, PCOS patients were divided into obese（n=54） and non-obese（n=66） groups. Based on Homa-IR, the patients were divided into insulin resistant（IR, n=49） and non-tR（n=71） groups. Results The rate of PAI-1 gene promoter region 4G/4G genotype was significantly higher in the PCQS patients than that in control group（P〈0.05）. The rate of 4G/4G genotype was significantly higher in the IR and non-obese groups than that in non-IR and obese groups （P〈0.05）. Conclusion The genotype 4G/4G in PAI-1 gene promoter region may be associated with the pathogenesis of PCOS in Zhejiang Han population, which may be involved in the development of IR and PCOS, especially in non-obese patients,
出处
《浙江医学》
CAS
2016年第8期527-530,共4页
Zhejiang Medical Journal
基金
浙江省科技计划项目(2009C33114)
