不同剂量富氢气生理盐水对实验性自身免疫性脑脊髓炎的治疗作用观察

Effects of hydrogen-rich saline against experimental autoimmune encephalomyelitis in SD rats

目的探讨富氢气生理盐水对实验性自身免疫性脑脊髓炎（EAE）大鼠的治疗作用及最佳剂量范围。方法用豚鼠脊髓匀浆与完全弗氏佐剂免疫SD大鼠制作EAE模型；造模成功后第1～20天大鼠腹腔注射不同剂量（0、3、6ml／kg）富氢气生理盐水，观察并记录大鼠体重、行为学评分；第21天处死大鼠取脑、脊髓作HE染色，检测脊髓IL-17、INF—γ表达水平以及血清超氧化物歧化酶（SOD）活性。结果6ml／kg富氢气生理盐水剂量组临床发病率较低，仅为30．0％；且发病后能抑制体重下降，最高临床评分与累积临床评分均最低，中枢组织炎症细胞浸润最少。3、6ml／kg剂量的富氢气生理盐水均能抑制EAE大鼠IL-17、INF—γ的释放，提高SOD活性；但6ml／kg剂量效果更佳。结论6ml／kg剂量的富氢气生理盐水可能对降低EAE大鼠临床发病率有积极作用，且能抑制发病大鼠体重下降，降低神经缺损行为学评分，并能促进SOD活性，从而抑制EAE。

Objective To investigate the effect of hydrogen-rich saline on experimental autoimmune encephalomyelitis （EAE）. Methods EAE was induced by injection of spinal cord homogenate of guinea pig with complete Freund＇s adjuvant （CFA） in SD rats. From the 1st day to the 20th day after EAE induced, the rats received peritoneal injection of hydrogen-rich saline （0, 3, 6ml/kg）. On the 21th day, all rats were sacrificed; paraffin sections of spine cord were prepared and stained with hematoxylin-eosin to detect inflammatory cell infiltration. The expression of IL-17 and INF- γ in the spinal cord was determined by ELISA and serum SOD activity was determined on the 10th, 15th and 20th after EAE induced. Results The incidence of EAE in hydrogen-rich saline 6ml/kg group was lowest （30.0%） among 3 groups. Hydrogen-rich saline 6ml/kg inhibited the weight reduction and reduced the neurobehavioral deficits scores. It also reduced the inflammatory cell infiltration in the spinal cord of EAE rats and the expression of IL-17 and INFγ, while increased the level of SOD. The degree of weight reduction was not reduced by given 3ml/kg hydrogen-rich saline, while it reduced the neurobehavioral deficits scores and the inflammatory cell infiltration in spinal cord and the expression of IL-17 and INF-γand up-regulated the SOD level. Conclusion Hydrogen-rich saline can reduce the severity of EAE, which may be associated with its antioxidant effect and the up-regulation of serum SOD.