桂红胶囊中银杏叶总黄酮醇苷的含量测定

Content Determination of Total Flavonoids Glycosides of Ginkgo Biloba in Guihong Capsule

目的：建立测定桂红胶囊中银杏叶总黄酮醇苷含量的方法。方法：用HPLC方法测定桂红胶囊中银杏叶总黄酮醇苷含量。以甲醇-0.4%磷酸（50：50）为流动相,检测波长为370 nm,流速为1.0 m L/min。结果：槲皮素在0.066~1.054μg范围内线性关系良好（r=0.9999,n=5）,山柰素在0.068~1.628μg范围内线性关系良好（r=0.9999,n=5）,异鼠李素在0.017~0.266μg范围内线性关系良好（r=0.9999,n=5）;平均加样回收率：槲皮素为99.60%（RSD=2.53%,n=6）,山柰素为99.00%（RSD=2.77%,n=6）,异鼠李素为99.68%（RSD=2.61%,n=6）。结论：该法操作简便,灵敏度高、重现性好,可用于桂红胶囊中银杏叶总黄酮醇苷的含量测定。

Objective：To establish a method for content determination of total flavones glycosides of ginkgo bfloba in Guihong capsule. Method：High performance liquid chromatography（HPLC） was applied to determine flavonoids glycosides. The mobile phase was methanol-phosphate acid （0.4%） （50 ： 50）, and the detective wavelength was 370 nm;the flow rate was 1.0 mL/min,Results ：the linear ranges of quercetin,kaempferide and isorhamnetin were 0.066- 0.527 μg, 0.068 N 0.814 μg （r=0.9999, n=5 ） and 0.017 - 0.133 μg, respectively. The average recoveries of these three compounds were 99.60%（RSD=2.53%,n=6）,99.00%（RSD=2.77%,n=6） and 99.68%（RSD=2.61% ,n=6）,respectively. Conclusion：This method was simple,sensitive,repeatable,and could be used for the content determination of total flavonoids glycosides in the ginkgo biloba extract.