大鼠脊髓半横断伤联合架桥模型制作及评估

Making and Assessment of Spinal Cord Hemisection Injury Joint Bridge Animal Model

目的:建立大鼠脊髓半横断伤联合架桥模型,为研究脊髓损伤提供动物模型。方法:制作大鼠脊髓半横断伤模型,然后取大鼠前肢正中神经,并于半横断伤两端行正中神经架桥术。术后4周,左心室灌注固定取材,免疫组化染色检测GFAP、RECA、NF-200;另一部分动物行单宁酸-氯化铁灌注;观察移植物内有无血管、血管内有无血流、血管与周边神经纤维的关系。结果:外周神经架桥后4周,移植正中神经贴合于脊髓背侧1/2。移植神经内有RECA阳性的血管存在,而且有血流可以到达移植物内部,且神经纤维(NF-200阳性)与星形胶质细胞(GFAP阳性)关系紧密。结论:大鼠脊髓半横断伤联合正中神经架桥术后,由宿主可以向移植物内长入新生血管,血管有利于神经纤维的存活及生长。本模型为较好的外周神经移植的存活模型,可为进一步的深入研究提供一定的依据。

Objective： To establish the animal model of hemisection of spinal cord injury joint bridge of rats for the study of spinal cord injury. Methods： The rat model ofhemisection of spinal cord injury was established, then making joint bridge by median nerve of rat＇s forelimb. Four weeks later, left ventricular of rats was perfused, fixed and coveraged. The expression of GFAP, RECA, NF-200 was detected by immunohistochemistry; the others rats were detected using tannic acid-ferric chloride perfused method. Finally, the situation of neovascularization in the grafts and the relation between neovascularization and peripheral nerve fibers was detected. Results： The re- sults of RECA and HE staining after perfusion with tannic acid-ferric chloride showed that the neovascularization was detectable in the grafts, and the blood could reach the inside of the grafts. Some NF-positive nerve fibers were co-localized with GFAP-positive im- munoreactivity. Conclusions： The neovascularization from host can reach the inside of graft after hemisection of spinal cord injury joint bridge by median nerve, which is beneficial to the survival and growth of nerve fibers. The animal model of spinal cord hemisection joint bridge could provide the basis for the survival of peripheral nerve and its further study.