细胞因子诱导的杀伤细胞对人肺腺癌细胞及其辐射抗性细胞的杀伤作用

Killing effect of cytokine-induced killer cells on human lung adenocarcinoma and its radiation resistant cell lines

目的探讨细胞因子诱导的杀伤细胞（CIK细胞）对人肺腺癌细胞A549及其辐射抗性细胞A549RR的杀伤作用。方法应用多种细胞因子在体外将健康志愿者外周血单个核细胞（PBMC）诱导形成具有杀伤活性的CIK细胞，采用流式细胞术检测细胞免疫表型。将CIK细胞分别作用于A549和A549RR细胞，采用CCK8法检测细胞吸光度（A）值并计算CIK细胞作用24h和48h对各组细胞的杀伤率。结果CIK细胞体外培养14d后，CD3^＋CD56^＋细胞所占比例为45．8％。CIK细胞对A549及A549RR细胞的杀伤率随着效靶比（5：1～40：1）的增高及作用时间的延长而增强（均P〈0．001）。在同一作用时间、相同效靶比时，CIK细胞对于A549和A549RR细胞的杀伤作用差异均无统计学意义（均P〉0．05）。结论CIK细胞对肺腺癌细胞及其辐射抗性细胞有很强杀伤作用，具有较高的临床应用价值。

Objective To study the killing effect of cytokine-induced killer cells （CIK cells） on human lung adenoearcinoma cell line （A549） and the lung adenoeareinoma＇s radiation resistant cell line （A549RR）. Methods Peripheral blood mononuclear cells （PBMC） of healthy volunteers were stimulated by different cytokines, and were induced into killer activity CIK cells. The phenotype of CIK cells were analyzed by flow eytometer. A549 and A549RR cell lines were cultured separately with the CIK cells. The absorbance value （A） of the cells was measured by CCK8, and the killing rates of all cells which were cultured for 24 and 48 hours with the CIK were calculated. Results The rate of CD3^＋ CD56^＋ cell was 45.8 % after culture for 14 d. The killing rates of CIK cells to lung adenocarcinoma A549 cells and its radiation resistant cells A549RR were increased with the rise of the ratio of effective cells to target （5：1-40：1） and the increasing of culturing time （all P 〈 0.001）. The killing effect of CIK to A549 and A549RR cells had no obvious difference in the same culturing time and the same ratio of effective cells to target（all P 〉 0.05）. Conclusion CIK cells have strong anti-tumor effect against lung adenocarcinoma and its radiation resistant cells with high clinical application value.