改良套管法大鼠肝移植模型的建立

Establishment of liver transplantation model with improved two-cuff in rat

目的建立大鼠原位肝移植模型，为肝移植相关问题的研究搭建一个良好的技术平台。方法在经典“双套管法”的基础上，综合多种改良方法建立大鼠肝移植模型制作方法：先经腹主动脉灌注后再结扎，单线连续外翻缝合肝上下腔静脉、术前术后补液、蚊式止血钳阻断门静脉和肝下腔静脉，观察大鼠术后2d成活率和2周生存率。结果供体大鼠手术时间为（35±2）min，修肝套管时间为（7．0±1．5）min，受体大鼠手术时间为（80±15）min，无肝期时间为（21±3）min，2d存活率为92％（46／50），2周存活率为86％（43／50）。结论改良套管法不仅能降低大鼠原位肝移植手术操作难度，而且能制作出均一、稳定、可靠的大鼠原位肝移植模型。

Objective To build rat orthotopic liver transplantation model and use this model as an experimental animal model for further research about liver transplantation. Methods Based on the classic ＂two-cuff＂ technique, a variety of skills were integrated,such as abdominal aorta perfusion first before ligation, suprahepatic inferior vena cava single continuous suture, preoperative and postoperative rehydration, hepatic portal vein and infrahepatic vena cava blocked by mosquito＂ s haemostatic forceps. At last, the postoperative survival rates of 2 days and 2 weeks were recorded. Results Donors＂ operation time was （35±2） min, cuffing time was （7.0±1.5） min, receptors＂ operation time was （80±15） min, anhepatic phase was （21±3） min. The 2-day survival rate was 92 % （46/50） and 2-week survival rate was 86 % （43/50）. Conclusion To some extent,the improved methods can not only reduce the difficulty of orthotopic liver transplantation operation, but also to create a uniform, stable and reliable orthotopic liver transplantation model.