E26转录因子和VEGF在卵巢上皮性肿瘤中的表达及意义

Expression of Ets-1 and VEGF in epithelial ovarian neoplasms and its clinicopathological significance

目的探讨E26转录因子(E26 transformation specific-1,Ets-1)及血管内皮生长因子(vascular endothelial growth factor,VEGF)在卵巢上皮性肿瘤中的表达及意义。方法在55例卵巢上皮性肿瘤以及12例正常卵巢组织中,采用免疫组化SP法检测其中Ets-1及VEGF蛋白的表达;原位杂交法检测组织中Ets-1 mRNA的表达。结果Ets-1及VEGF在卵巢上皮性癌中的表达显著高于正常、良性及交界组(P<0.01)。FIGOⅢ-Ⅳ期组中Ets-1以及VEGF蛋白的表达均高于FIGOⅠ-Ⅱ早期组(P<0.05);肿瘤细胞低分化组中Ets-1的表达明显高于中高分化组(P<0.01);有淋巴结转移组中两者表达高于无淋巴结转移组(P<0.05);Ets-1蛋白和m RNA的表达显著相关(rs为0.609,P<0.01);肿瘤组织中Ets-1和VEGF的表达密切相关(rs=0.288,P<0.05)。结论 Ets-1和VEGF是卵巢上皮性肿瘤中重要的促血管生成因子,与肿瘤的发展转移及患者的预后关系密切。

Objective To examine the expression of E26 transformation specific-1 （Ets-1） and vascular endothelial growth factor（VEGF） in epithelial ovarian neoplasm, and its clinicopathological significance. Methods The expression of Ets-1 and VEGF proteins were detected by immun- ohistochemistry, and the expression of Ets-1 mRNA was detected by in situ hybridization in 55 tissue samples of epithelial ovarian neoplasms and 12 samples of pericancerous ovarian tissue. Results Serous cystadenocarcinoma had higher expression of both Ets-1 and VEGF than normal ovarian tissue, serous cystadenoma and borderline serous cystadenoma（ P 〈 0.01 ）. The expression of Ets-1 and VEGF in ovarian carcinoma Ⅰ-Ⅱ stage was lower than that in carcinomaⅢ-Ⅳ stage（ P 〈 0. 05 ） ; expression of Ets-1 in low histological grade ovarian carcinoma was higher than that in middle and high histological grade carcinoma （ P 〈 0.01 ） ; the expression of Ets-1 and VEGF in ovarian carcinoma without lymph nodes metastasis was lower than that in those with metastases （ P 〈 0.05 ）. The expression of Ets-1 mRNA was positively correlated with expression of Ets-1 and VEGF proteins （ rs = 0. 609, rs = 0. 288, respectively, both P 〈 0.05 ）. Conclusion The results indicate that Ets-1 and VEGF may be involved in the development and metastases of epithelial ovarian neoplasms.