放射线促进脂质体转染人直肠癌HR-8348细胞的实验研究

Enhancement of ionizing radiation on liposome-mediated gene delivery in human rectal cancer HR-8348 cells

目的 观察放射线对于脂质体转染的影响 ,探索提高脂质体转染效率的方法。 方法选出 0、2、4、8Gy不同照射剂量 ,分别于转染pEGFP N1荧光表达质粒前 1h照射 ,36h倒置荧光显微镜下观察荧光表达细胞和计数本底细胞 ,计算转染效率。 结果 在HR 834 8细胞中 ,放射线可以促进脂质体对pEGFP N1质粒的转染效率 ,应用 0、2、4、8Gy照射剂量转染效率分别为 2 1 32 %、6 2 17%、6 8 0 0 %、77 78% ,放射组与未放射组之间差异存在非常显著性意义 (χ2 =131 7,P <0 0 1) ,照射后脂质体转染效率可与病毒载体转染效率接近。 结论 利用放射线可以明显促进脂质体的转染效率。

Objectives To investigate the effect of ionizing radiation on liposome-mediated gene delivery and find out a way to improve gene transfection. Methods Prior to liposome transfection, HR-8348 cells were irradiated at doses of 0,2,4,8 Gy selected according to the surviving fraction line of HR-8348 cells after different dosage of radiation. After 36 h of liposome transfection, green fluorocytes were counted. The transfection efficiency was figured out and compared with each other. Results The transfection efficiency of liposome-mediated gene delivery was 21.32%, 62.17%,68.00%,77.78% at the dose of 0,2,4,8 Gy respectively and the clinical dose (2 Gy) was as high as 62.17%. Combined radiation and liposome-mediated gene delivery achieved the approximate transfection efficiency of virus vector. Conclusion Ionizing radiation can improve the transfection efficiency of liposome-mediated gene delivery markedly and it is expected to treat human malignancy with liposome-mediated gene delivery combined with radiation.