盐胁迫下内生真菌感染对中华羊茅幼苗生长的影响

Effect of Epichloё on Seedling Growth of Festuca sinensis Under Salt Stress

以带菌（E＋）和不带菌（E-）的中华羊茅为试材,采用培养皿纸上发芽法,研究了不同NaCl浓度对中华羊茅幼苗苗长、根长、苗鲜重和根鲜重的影响,以期为内生真菌对宿主耐盐性的作用提供参考依据。结果表明：E＋幼苗中,盐浓度为1.0-8.0mmol/L时显著促进苗长、根长和苗鲜重的增加（P〈0.05）,而1.0-2.0mmol/L盐浓度对根鲜重有明显增效作用（P〈0.05）;E-幼苗中,1.0-2.0mmol/L盐浓度显著增加根长（P〈0.05）,2.0-8.0mmol/L盐浓度明显增加苗鲜重（P〈0.05）,盐浓度8.0mmol/L显著抑制根的伸长和鲜重（P〈0.05）。与E-幼苗相比,对照条件下除内生真菌显著增加苗鲜重外（P〈0.05）,其它所测生长指标均无明显差异性;在1.0-8.0mmol/L盐浓度下内生真菌显著增加根长和苗鲜重（P〈0.05）,在1.0-4.0mmol/L盐浓度下内生真菌明显促进苗长（P〈0.05）,在2.0mmol/L或8.0mmol/L盐浓度下内生真菌明显增加根鲜重（P〈0.05）;苗长与根长或苗鲜重相关系数较高,且内生真菌能提高其相关系数。

Using seedlings growth of Festuca sinensis infected-endophyte（E＋）and uninfected-endophyte（E-）as material,the effects of NaCl concentrations on seedling length,root length,seedling fresh weight and root fresh weight of Festuca sinensis were studied for providing theoretical basis for the effects of Epichloendophyte infection on salt stress by germination method of Petri plates.The results showed that in E＋seedlings salt concentrations ranged from 1.0mmol/L to 8.0mmol/L promoted significantly seedling length,root length and seedling fresh weight（P〈0.05）,and salt concentrations between 1.0 mmol/L and 2.0 mmol/L increased significantly root fresh weight（P〈0.05）.In E-seedlings root length was increased under salt concentration ranging from 1.0mmol/L to 2.0mmol/L（P〈0.05）,seedling fresh weight increased significantly under all salt concentration except 1.0mmol/L NaCl（P〈0.05）,and root length and fresh weight were significantly inhibited by 8.0mmol/L NaCl（P〈0.05）.Compared with E-seedlings,E＋ones had higher seedling fresh weight under control conditions,E＋ones had higher root length and seedling fresh weight under all salt concentration（P〈0.05）,E＋ones had higher seedling length under salt concentration ranging from1.0mmol/L to 4.0mmol/L（P〈0.05）,and E＋ones had higher root fresh weight under 2.0mmol/L or 8.0mmol/L NaCl（P〈0.05）.There were high positive correlation between seedling length and root length,and between seedling length and fresh weight,which were higher in E＋seedlings.