膀胱癌端粒酶活性与P53、c-myc、bc1-2蛋白表达的检测及其临床意义

Detection of telomerase activity, P53, c-myc,bcl-2 protein in bladder cancer and its clinical significance

目的 探讨端粒酶活性与P53、c-myc、bcl- 2基因在膀胱癌发生发展中的作用以及端粒酶活性与 3种与癌相关基因之间的关系。方法 采用端粒酶重复序列扩增法及酶联免疫吸附法和电脉法检测 58例膀胱癌、1 0例癌旁粘膜及 1 0例正常膀胱粘膜的端粒酶活性 ,采用免疫组织化学S -P法检测P53、c-myc、bcl- 2蛋白的表达。结果 膀胱癌组织端粒酶活性、P53、c -myc和bcl- 2蛋白阳性率分别为 86 .2 %、60 .3 %、68.9%和 81 % ,癌旁组织端粒酶活性、c -myc和bcl- 2蛋白阳性率为 1 0 %、1 0 %和 2 0 % ,P53蛋白为阴性 ,正常组织中端粒酶活性、P53、c-myc蛋白为阴性 ,bcl- 2蛋白阳性率为 1 0 % ,膀胱癌组织显著高于癌旁及正常组织 (P <0 .0 1 ) ,不同病理分级、临床分期之间端粒酶活性无显著性差异 (P >0 .0 5) ,P53、c -myc和bcl- 2异常表达有显著性差异 (P <0 .0 1或P <0 .0 5)。端粒酶活性与P53、c-myc、bcl- 2蛋白表达之间无明显相关性 (P >0 .0 5)。结论 端粒酶活化P53、c-myc和bcl- 2基因均参与膀胱癌的发生发展过程 ,联合检测有助于膀胱癌的早期诊断 ,端粒酶与P53、c-myc和bcl- 2无明显相关性 。

Objectives To study the role of telomerase activity,P53,c-myc and bcl-2 in patholgenesis and progression of bladder transitional cell carcinoma (TCC) and the relation among them.Methods Telomerase activity was examined by TRAP-ELISA and the expression of P53,c-myc and bcl-2 protein was detected by immunohistochemical S-P method in 58 cases of TCC,10 cases of the adjacent tissues and 10 cases of normal bladder tissues.Results The positive rate of telomerase activity,P53,c-myc and bcl-2 protein were 86.2%?60.3%?68.9%?and 81% respectively in TCC and obviously higher than in the adjacent and normal bladder tissues(P<0.01).No significant correlation was observed between the positive rate of telomerase activity and the pathologic grade and the clinical stage(P>0.05),tbe expression of P53,c-myc and bcl-2 protein were found to be positively correlated to the pathologic grade and the clinical stage (P<0.01or P<0.05).No significant correlation between the telomerase activity and the expression of P53, c-myc and bcl-2 protein was found(P>0.05).Conclusions The telomerase activity and the abnormal expression of P53, c-myc and bcl-2 protein may be involved in the pathogensis and development of bladder transitional cell carcinoma, will be helpful for diagnosis and treatmeat of TCC. There are no significant correlation between telomerase, P53, c-myc and bcl-2 indicate that multiple genes may be involved in the pathogensis of TCC.