通络醒脑泡腾片对MID模型大鼠血液流变学,iNOS,VEGF及LDH-5的影响

Effects of Tongluo Xingnao effervescent tablets on blood rheology,iNOS,VEGF and LDH-5 in MID rats

考察通络醒脑泡腾片对多发脑梗死性痴呆(multi-infarct dementia,MID)模型大鼠血液流变学,i NOS,VEGF及LDH-5影响。采用微血栓栓塞法制备MID大鼠模型,将造模成功大鼠50只随机分为模型对照组,甲磺酸双氢麦角毒碱片(喜得镇)组(0.7 mg·kg^(-1)),通络醒脑泡腾片高、中、低剂量组(7.56,3.78,1.59 g·kg^(-1)),另取10只假手术动物作为平行对照组,连续灌胃90 d。运用Morris水迷宫检测大鼠的学习记忆能力;腹主动脉取血测定不同切变率下的全血黏度和红细胞聚集指数;采用ELISA法测定血清i NOS和VEGF的含量;采用免疫组化和图像分析技术测定MID大鼠海马LDH-5的表达。结果显示通络醒脑泡腾片能明显缩短MID模型大鼠的逃避潜伏期,增加逃逸平台进入次数,延长中环滞留时间,在1,5,30,200 S-14个切变率下,也能显著降低模型大鼠的全血黏度,红细胞聚集指数以及血清i NOS,VEGF含量和LDH-5平均光密度,与模型对照组比较有统计学意义(P<0.05)。结果表明通络醒脑泡腾片能提高MID模型大鼠学习记忆能力,改善外周血液流变学,降低i NOS,VEGF的含量及海马LDH-5表达,进而改善脑组织能量供应。

The study was to explore effects of Tongluo Xingnao effervescent tablets on the blood rheology,i NOS,VEGF and LDH-5 in multi-infarct dementia（ MID） model rats. Establish MID model rats were induced by microthrombosis,from which 50 successful model rats were randomly divided into five groups,such as the model control group,the dihydroergotoxine mesylate tablets（ hydergine）group（ 0. 7 mg·kg^（-1））,Tongluo Xingnao effervescent tablets high-dose,medium-dose and low-dose groups（ 7. 56,3. 78,1. 89 g·kg^（-1））. Another ten rats in the sham group were randomly selected as the parallel control group. Each group was orally administered with drugs for 90 days. The learning and memory ability was evaluated with the Morris water maze test,while the whole blood viscosity and the erythrocyte aggregation index derived from abdominal aorta were measured in different shear rates. In addition,the levels of VEGF and i NOS in the serum were determined by ELISA kits. The expression of LDH-5 in hippocampus of rats was measured with immunohistochemistry and image quantitative analysis. The result showed that Tongluo Xingnao effervescent tablets notably decreased the escape latency of MID model rats,increased times of entering into the escape platform and prolonged retention time in medium ring,meanwhile the whole blood viscosity in MID model rats was also notably reduced in four shear rates,i. e. 1,5,30,200 S- 1,erythrocyte aggregation index,serum VEGF and i NOS,and average optical density value of LDH-5,with a statistically significant differences compared with the model control group（ P 0. 05）. In conclusion,Tongluo Xingnao effervescent tablets could improve the ability of learning and memory of MID model rats and the blood rheology,reduce the level of i NOS,VEGF and the expression of LDH-5,and then improved the brain energy supply.